In vitro evaluation of the activity of terpenes and cannabidiol against human coronavirus E229

Lior Chatow; Adi Nudel; Iris Nesher; David Hayo Hemo; Perri Rozenberg; Hanna Voropaev; Ilan Winkler; Ronnie Levy; Zohar Kerem; Zohara Yaniv; Nadav Eyal

doi:10.3390/life11040290

In vitro evaluation of the activity of terpenes and cannabidiol against human coronavirus E229

Lior Chatow^*, Adi Nudel, Iris Nesher, David Hayo Hemo, Perri Rozenberg, Hanna Voropaev, Ilan Winkler, Ronnie Levy, Zohar Kerem, Zohara Yaniv, Nadav Eyal

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

The activity of a new, terpene-based formulation, code-named NT-VRL-1, against Human Coronavirus (HCoV) strain 229E was evaluated in human lung fibroblasts (MRC-5 cells), with and without the addition of cannabidiol (CBD). The main constituents in the terpene formulation used for the experiment were beta caryophyllene, eucalyptol, and citral. The tested formulation exhibited an antiviral effect when it was pre-incubated with the host cells prior to virus infection. The combination of NT-VRL-1 with CBD potentiated the antiviral effect better than the positive controls pyrazofurin and glycyrrhizin. There was a strong correlation between the quantitative results from a cell-viability assay and the cytopathic effect seen under the microscope after 72 h. To the best of our knowledge, this is the first report of activity of a combination of terpenes and CBD against a coronavirus.

Original language	English
Article number	290
Journal	Life
Volume	11
Issue number	4
DOIs	https://doi.org/10.3390/life11040290
State	Published - Apr 2021
Externally published	Yes

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Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

Antiviral
CBD
COVID-19
Cannabis
HCoV-229E
Terpenes

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10.3390/life11040290

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title = "In vitro evaluation of the activity of terpenes and cannabidiol against human coronavirus E229",

abstract = "The activity of a new, terpene-based formulation, code-named NT-VRL-1, against Human Coronavirus (HCoV) strain 229E was evaluated in human lung fibroblasts (MRC-5 cells), with and without the addition of cannabidiol (CBD). The main constituents in the terpene formulation used for the experiment were beta caryophyllene, eucalyptol, and citral. The tested formulation exhibited an antiviral effect when it was pre-incubated with the host cells prior to virus infection. The combination of NT-VRL-1 with CBD potentiated the antiviral effect better than the positive controls pyrazofurin and glycyrrhizin. There was a strong correlation between the quantitative results from a cell-viability assay and the cytopathic effect seen under the microscope after 72 h. To the best of our knowledge, this is the first report of activity of a combination of terpenes and CBD against a coronavirus.",

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author = "Lior Chatow and Adi Nudel and Iris Nesher and Hemo, {David Hayo} and Perri Rozenberg and Hanna Voropaev and Ilan Winkler and Ronnie Levy and Zohar Kerem and Zohara Yaniv and Nadav Eyal",

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doi = "10.3390/life11040290",

language = "אנגלית",

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AU - Chatow, Lior

AU - Nudel, Adi

AU - Nesher, Iris

AU - Hemo, David Hayo

AU - Rozenberg, Perri

AU - Voropaev, Hanna

AU - Winkler, Ilan

AU - Levy, Ronnie

AU - Kerem, Zohar

AU - Yaniv, Zohara

AU - Eyal, Nadav

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N2 - The activity of a new, terpene-based formulation, code-named NT-VRL-1, against Human Coronavirus (HCoV) strain 229E was evaluated in human lung fibroblasts (MRC-5 cells), with and without the addition of cannabidiol (CBD). The main constituents in the terpene formulation used for the experiment were beta caryophyllene, eucalyptol, and citral. The tested formulation exhibited an antiviral effect when it was pre-incubated with the host cells prior to virus infection. The combination of NT-VRL-1 with CBD potentiated the antiviral effect better than the positive controls pyrazofurin and glycyrrhizin. There was a strong correlation between the quantitative results from a cell-viability assay and the cytopathic effect seen under the microscope after 72 h. To the best of our knowledge, this is the first report of activity of a combination of terpenes and CBD against a coronavirus.

AB - The activity of a new, terpene-based formulation, code-named NT-VRL-1, against Human Coronavirus (HCoV) strain 229E was evaluated in human lung fibroblasts (MRC-5 cells), with and without the addition of cannabidiol (CBD). The main constituents in the terpene formulation used for the experiment were beta caryophyllene, eucalyptol, and citral. The tested formulation exhibited an antiviral effect when it was pre-incubated with the host cells prior to virus infection. The combination of NT-VRL-1 with CBD potentiated the antiviral effect better than the positive controls pyrazofurin and glycyrrhizin. There was a strong correlation between the quantitative results from a cell-viability assay and the cytopathic effect seen under the microscope after 72 h. To the best of our knowledge, this is the first report of activity of a combination of terpenes and CBD against a coronavirus.

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In vitro evaluation of the activity of terpenes and cannabidiol against human coronavirus E229

Abstract

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Keywords

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