In vitro produced monoclonal rheumatoid factor: Purification, radiolabel, and possible applications

Michael Steinitz*, Norio Koide, Gad Spira, Sara Tamir, George Klein

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

A human monoclonal rheumatoid factor (IgM, λ) produced in vitro by Epstein-Barr virus (EBV) immortalized cell line was purified from cell culture medium. Purification, based on the binding of this antibody to protein A, was carried out by adsorption on protein A-Sepharose and elution with low pH buffer. The eluted preparation (×400 purified) was radiolabeled. The specific binding of the purified rheumatoid factor (RF) to immune complexes was kept intact. This unique human monoclonal antibody is applied as a universal reagent to detect low titers of antibodies directed toward cellular antigens.

Original languageEnglish
Pages (from-to)205-214
Number of pages10
JournalCellular Immunology
Volume69
Issue number2
DOIs
StatePublished - 15 May 1982
Externally publishedYes

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