TY - JOUR
T1 - In vitro produced monoclonal rheumatoid factor
T2 - Purification, radiolabel, and possible applications
AU - Steinitz, Michael
AU - Koide, Norio
AU - Spira, Gad
AU - Tamir, Sara
AU - Klein, George
PY - 1982/5/15
Y1 - 1982/5/15
N2 - A human monoclonal rheumatoid factor (IgM, λ) produced in vitro by Epstein-Barr virus (EBV) immortalized cell line was purified from cell culture medium. Purification, based on the binding of this antibody to protein A, was carried out by adsorption on protein A-Sepharose and elution with low pH buffer. The eluted preparation (×400 purified) was radiolabeled. The specific binding of the purified rheumatoid factor (RF) to immune complexes was kept intact. This unique human monoclonal antibody is applied as a universal reagent to detect low titers of antibodies directed toward cellular antigens.
AB - A human monoclonal rheumatoid factor (IgM, λ) produced in vitro by Epstein-Barr virus (EBV) immortalized cell line was purified from cell culture medium. Purification, based on the binding of this antibody to protein A, was carried out by adsorption on protein A-Sepharose and elution with low pH buffer. The eluted preparation (×400 purified) was radiolabeled. The specific binding of the purified rheumatoid factor (RF) to immune complexes was kept intact. This unique human monoclonal antibody is applied as a universal reagent to detect low titers of antibodies directed toward cellular antigens.
UR - http://www.scopus.com/inward/record.url?scp=0019976605&partnerID=8YFLogxK
U2 - 10.1016/0008-8749(82)90067-3
DO - 10.1016/0008-8749(82)90067-3
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C2 - 7105186
AN - SCOPUS:0019976605
SN - 0008-8749
VL - 69
SP - 205
EP - 214
JO - Cellular Immunology
JF - Cellular Immunology
IS - 2
ER -