In vitro regeneration of activated rat peritoneal mast cells cocultured with 3T3 fibroblasts

Francesca Levi-Schaffer*, Naomi Riesel

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Rat peritoneal mast cells (MC) cocultured with 3T3 fibroblasts (MC/3T3) were activated by compound 48 80 to release most of their histamine content (∼90%). These activated MC could be kept viable in vitro for as long as 2 weeks, during which their regeneration had been followed. As early as 3-4 hr following activation, the MC started reacquiring their resting morphology. In 1-2 weeks they were more densely granulated grossly resembling nonactivated, control MC. Reactivation of MC/3T3 with compound 48 80 on Day 7 or 14 of culture resulted in a percentage histamine release which was similar to that of Day 0, and to control MC/3T3, indicating that these activated MC were functionally responsive. Activated MC/3T3 could partially resynthesize histamine during the 14 days of culture following activation. On Day 7 their histamine content was ∼23% and on Day 14 ∼40% of the initial amount they had on Day 0, prior to activation. Thus, MC/3T3 activated by compound 48 80 in vitro can regenerate by resuming their initial morphology, becoming responsive to a subsequent reactivation and resynthesizing histamine.

Original languageAmerican English
Pages (from-to)30-40
Number of pages11
JournalCellular Immunology
Volume119
Issue number1
DOIs
StatePublished - Mar 1989

Bibliographical note

Funding Information:
This work was partially supported by a grant from the Israeli Ministry of Absorption, by a grant from the Joint Research Fund of the Hebrew University and Hadassah, and by Grant No. 86-00 154/ 1 from the USA-Israel Binational Foundation.

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