In Vitro Storage of Functional Sperm at Room Temperature in Zebrafish and Medaka

Kazumasa Takemoto, Toshiya Nishimura, Toshihiro Kawasaki, Yukiko Imai, Karine Levy, Neta Hart, Ivan Olaya, Sean M. Burgess, Yaniv M. Elkouby, Minoru Tanaka, Noriyoshi Sakai*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


The longevity of sperm in teleost such as zebrafish and medaka is short when isolated even in saline-balanced solution at a physiological temperature. In contrast, some internal fertilizers exhibit the long-term storage of sperm, >10 months, in the female reproductive tract. This evidence implies that sperm in teleost possesses the ability to survive for a long time under suitable conditions; however, these conditions are not well understood. In this study, we show that the sperm of zebrafish can survive and maintain fertility in L-15-based storage medium supplemented with bovine serum albumin, fetal bovine serum, glucose, and lactic acid for 28 days at room temperature. The fertilized embryos developed to normal fertile adults. This storage medium was effective in medaka sperm stored for 7 days at room temperature. These results suggest that sperm from external fertilizer zebrafish and medaka has the ability to survive for at least 4 and 1 week, respectively, in the body fluid-like medium at a physiological temperature. This sperm storage method allows researchers to ship sperm by low-cost methods and to investigate key factors for motility and fertile ability in those sperm.

Original languageAmerican English
Pages (from-to)229-235
Number of pages7
Issue number6
StatePublished - 1 Dec 2023

Bibliographical note

Publisher Copyright:
© Mary Ann Liebert, Inc.


  • lactic acid
  • medaka
  • sperm storage
  • zebrafish


Dive into the research topics of 'In Vitro Storage of Functional Sperm at Room Temperature in Zebrafish and Medaka'. Together they form a unique fingerprint.

Cite this