In vitro synthesis of antibodies to acetylcholine receptor by Epstein-Barr virus-stimulated B-lymphocytes derived from patients with myasthenia gravis

Talma Brenner; Yael Timore; Itzhak Wirguin; Oded Abramsky; Michael Steinitz

doi:10.1016/0165-5728(89)90119-7

In vitro synthesis of antibodies to acetylcholine receptor by Epstein-Barr virus-stimulated B-lymphocytes derived from patients with myasthenia gravis

Talma Brenner^*, Yael Timore, Itzhak Wirguin, Oded Abramsky, Michael Steinitz

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Peripheral blood lymphocytes and B-cells were obtained from patients with myasthenia gravis and stimulated in vitro with either pokeweed mitogen or Epstein-Barr virus (EBV), respectively. EBV stimulation of B-cells caused a production of antibodies to acetylcholine receptor in 15 of the 25 myasthenia gravis patients: the EBV stimulation of B-cells was more effective in this regard than the pokeweed mitogen stimulation of peripheral blood lymphocytes. The in vitro synthesis of anti-acetylcholine receptor antibodies was found to be positively correlated with both the patient's sera antibody titers and with the disease severity.

Original language	English
Pages (from-to)	217-222
Number of pages	6
Journal	Journal of Neuroimmunology
Volume	24
Issue number	3
DOIs	https://doi.org/10.1016/0165-5728(89)90119-7
State	Published - Oct 1989

Keywords

Anti-acetylcholine receptor antibody
Autoantibody, production in vitro
Epstein-Barr virus-transformed B lymphocyte
Myasthenia gravis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/0165-5728(89)90119-7

Cite this

@article{bc561f77dfa74b4b884dba1ce489c501,

title = "In vitro synthesis of antibodies to acetylcholine receptor by Epstein-Barr virus-stimulated B-lymphocytes derived from patients with myasthenia gravis",

abstract = "Peripheral blood lymphocytes and B-cells were obtained from patients with myasthenia gravis and stimulated in vitro with either pokeweed mitogen or Epstein-Barr virus (EBV), respectively. EBV stimulation of B-cells caused a production of antibodies to acetylcholine receptor in 15 of the 25 myasthenia gravis patients: the EBV stimulation of B-cells was more effective in this regard than the pokeweed mitogen stimulation of peripheral blood lymphocytes. The in vitro synthesis of anti-acetylcholine receptor antibodies was found to be positively correlated with both the patient's sera antibody titers and with the disease severity.",

keywords = "Anti-acetylcholine receptor antibody, Autoantibody, production in vitro, Epstein-Barr virus-transformed B lymphocyte, Myasthenia gravis",

author = "Talma Brenner and Yael Timore and Itzhak Wirguin and Oded Abramsky and Michael Steinitz",

year = "1989",

month = oct,

doi = "10.1016/0165-5728(89)90119-7",

language = "אנגלית",

volume = "24",

pages = "217--222",

journal = "Journal of Neuroimmunology",

issn = "0165-5728",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - In vitro synthesis of antibodies to acetylcholine receptor by Epstein-Barr virus-stimulated B-lymphocytes derived from patients with myasthenia gravis

AU - Brenner, Talma

AU - Timore, Yael

AU - Wirguin, Itzhak

AU - Abramsky, Oded

AU - Steinitz, Michael

PY - 1989/10

Y1 - 1989/10

N2 - Peripheral blood lymphocytes and B-cells were obtained from patients with myasthenia gravis and stimulated in vitro with either pokeweed mitogen or Epstein-Barr virus (EBV), respectively. EBV stimulation of B-cells caused a production of antibodies to acetylcholine receptor in 15 of the 25 myasthenia gravis patients: the EBV stimulation of B-cells was more effective in this regard than the pokeweed mitogen stimulation of peripheral blood lymphocytes. The in vitro synthesis of anti-acetylcholine receptor antibodies was found to be positively correlated with both the patient's sera antibody titers and with the disease severity.

AB - Peripheral blood lymphocytes and B-cells were obtained from patients with myasthenia gravis and stimulated in vitro with either pokeweed mitogen or Epstein-Barr virus (EBV), respectively. EBV stimulation of B-cells caused a production of antibodies to acetylcholine receptor in 15 of the 25 myasthenia gravis patients: the EBV stimulation of B-cells was more effective in this regard than the pokeweed mitogen stimulation of peripheral blood lymphocytes. The in vitro synthesis of anti-acetylcholine receptor antibodies was found to be positively correlated with both the patient's sera antibody titers and with the disease severity.

KW - Anti-acetylcholine receptor antibody

KW - Autoantibody, production in vitro

KW - Epstein-Barr virus-transformed B lymphocyte

KW - Myasthenia gravis

UR - http://www.scopus.com/inward/record.url?scp=0024468527&partnerID=8YFLogxK

U2 - 10.1016/0165-5728(89)90119-7

DO - 10.1016/0165-5728(89)90119-7

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 2553772

AN - SCOPUS:0024468527

SN - 0165-5728

VL - 24

SP - 217

EP - 222

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

IS - 3

ER -

In vitro synthesis of antibodies to acetylcholine receptor by Epstein-Barr virus-stimulated B-lymphocytes derived from patients with myasthenia gravis

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this