TY - JOUR
T1 - In vitro synthesis of antibodies to myelin antigens by Epstein-Barr virus-transformed B lymphocytes from patients with neurologic disorders
AU - Wirguin, Itzhak
AU - Brenner, Talma
AU - Steinitz, Michael
AU - Abramsky, Oded
PY - 1991/7
Y1 - 1991/7
N2 - Anti-myelin antibodies can be found in sera from patients with neurologic disorders of suspected immune-mediated pathogenesis such as multiple sclerosis and inflammatory polyneuropathies. However, the specificity of these findings is controversial. In the present study, in vitro synthesis of antibodies to myelin components was compared to their presence in sera in diverse neurological disorders. Epstein-Barr virus was used to activate B lymphocytes for in vitro antibody production. Anti-myelin basic protein and anti-galactocerebroside antibodies were secreted in vitro by B lymphocytes derived from patients with neurological disorders of various etiologies and pathogenetic mechanisms. Anti-myelin basic protein antibodies were detected in many more cell culture supernatants than in sera from the same patients. In vitro secretion of antibodies to myelin antigens, as well as the presence of these antibodies in body fluids, are apparently non-specific for disease type and may be secondary to neural tissue damage.
AB - Anti-myelin antibodies can be found in sera from patients with neurologic disorders of suspected immune-mediated pathogenesis such as multiple sclerosis and inflammatory polyneuropathies. However, the specificity of these findings is controversial. In the present study, in vitro synthesis of antibodies to myelin components was compared to their presence in sera in diverse neurological disorders. Epstein-Barr virus was used to activate B lymphocytes for in vitro antibody production. Anti-myelin basic protein and anti-galactocerebroside antibodies were secreted in vitro by B lymphocytes derived from patients with neurological disorders of various etiologies and pathogenetic mechanisms. Anti-myelin basic protein antibodies were detected in many more cell culture supernatants than in sera from the same patients. In vitro secretion of antibodies to myelin antigens, as well as the presence of these antibodies in body fluids, are apparently non-specific for disease type and may be secondary to neural tissue damage.
KW - Anti-galactocerebroside antibodies
KW - Anti-myelin basic protein antibodies
KW - Epstein-Barr virus-transformed B lymphocytes
KW - In vitro antibody production
UR - http://www.scopus.com/inward/record.url?scp=0025772364&partnerID=8YFLogxK
U2 - 10.1016/0022-510X(91)90221-R
DO - 10.1016/0022-510X(91)90221-R
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C2 - 1717665
AN - SCOPUS:0025772364
SN - 0022-510X
VL - 104
SP - 92
EP - 96
JO - Journal of the Neurological Sciences
JF - Journal of the Neurological Sciences
IS - 1
ER -