In vivo imaging of juxtaglomerular neuron turnover in the mouse olfactory bulb

Adi Mizrahi*, Jing Lu, Ryan Irving, Guoping Feng, Lawrence C. Katz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

53 Scopus citations


As a consequence of adult neurogenesis, the olfactory bulb (OB) receives a continuous influx of newborn neurons well into adulthood. However, their rates of generation and turnover, the factors controlling their survival, and how newborn neurons intercalate into adult circuits are largely unknown. To visualize the dynamics of adult neurogenesis, we produced a line of transgenic mice expressing GFP in ≈70% of juxtaglomerular neurons (JGNs), a population that undergoes adult neurogenesis. Using in vivo two-photon microscopy, time-lapse analysis of identified JGN cell bodies revealed a neuronal turnover rate of ≈3% of this population per month. Although new neurons appeared and older ones disappeared, the overall number of JGNs remained constant. This approach provides a dynamic view of the actual appearance and disappearance of newborn neurons in the vertebrate central nervous system, and provides an experimental substrate for functional analysis of adult neurogenesis.

Original languageAmerican English
Pages (from-to)1912-1917
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number6
StatePublished - 15 Feb 2006


  • Neurogenesis
  • Olfaction


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