In vivo optical recordings of ion dynamics in mouse corneal primary nociceptive terminals

Devora Gershon, Robert H. Negev-Goldstein, Lama Abd al Razzaq, Shaya Lev, Alexander M. Binshtok*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

This protocol aims to measure ion dynamics in nociceptive terminal endings in intact mice in vivo. We describe viral injection of GCaMP6s + RFP into trigeminal ganglia (TG) of mice, followed by calcium imaging of corneal nociceptive terminals that express GCaMP6s and RFP. This fast and high-resolution optical recording technique enables studying a nociceptive terminal's functional molecular network in physiological and pathological conditions. This platform can be applied to studying the physiology of terminals of other neurons. For complete details on the use and execution of this protocol, please refer to Goldstein et al. (2019).

Original languageAmerican English
Article number101224
JournalSTAR Protocols
Volume3
Issue number1
DOIs
StatePublished - 18 Mar 2022

Bibliographical note

Publisher Copyright:
© 2022 The Author(s)

Keywords

  • Microscopy
  • Model Organisms
  • Neuroscience

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