In vivo optical recordings of ion dynamics in mouse corneal primary nociceptive terminals

Devora Gershon; Robert H. Negev-Goldstein; Lama Abd al Razzaq; Shaya Lev; Alexander M. Binshtok

doi:10.1016/j.xpro.2022.101224

In vivo optical recordings of ion dynamics in mouse corneal primary nociceptive terminals

Devora Gershon, Robert H. Negev-Goldstein, Lama Abd al Razzaq, Shaya Lev, Alexander M. Binshtok^*

^*Corresponding author for this work

Department of Medical Neurobiology

Research output: Contribution to journal › Article › peer-review

Abstract

This protocol aims to measure ion dynamics in nociceptive terminal endings in intact mice in vivo. We describe viral injection of GCaMP6s + RFP into trigeminal ganglia (TG) of mice, followed by calcium imaging of corneal nociceptive terminals that express GCaMP6s and RFP. This fast and high-resolution optical recording technique enables studying a nociceptive terminal's functional molecular network in physiological and pathological conditions. This platform can be applied to studying the physiology of terminals of other neurons. For complete details on the use and execution of this protocol, please refer to Goldstein et al. (2019).

Original language	English
Article number	101224
Journal	STAR Protocols
Volume	3
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2022.101224
State	Published - 18 Mar 2022

Bibliographical note

Publisher Copyright:
© 2022 The Author(s)

Keywords

Microscopy
Model Organisms
Neuroscience

Access to Document

10.1016/j.xpro.2022.101224

Cite this

@article{252fdaa0e9f64d7e871428ee222bc91d,

title = "In vivo optical recordings of ion dynamics in mouse corneal primary nociceptive terminals",

abstract = "This protocol aims to measure ion dynamics in nociceptive terminal endings in intact mice in vivo. We describe viral injection of GCaMP6s + RFP into trigeminal ganglia (TG) of mice, followed by calcium imaging of corneal nociceptive terminals that express GCaMP6s and RFP. This fast and high-resolution optical recording technique enables studying a nociceptive terminal's functional molecular network in physiological and pathological conditions. This platform can be applied to studying the physiology of terminals of other neurons. For complete details on the use and execution of this protocol, please refer to Goldstein et al. (2019).",

keywords = "Microscopy, Model Organisms, Neuroscience",

author = "Devora Gershon and Negev-Goldstein, {Robert H.} and {Abd al Razzaq}, Lama and Shaya Lev and Binshtok, {Alexander M.}",

note = "Publisher Copyright: {\textcopyright} 2022 The Author(s)",

year = "2022",

month = mar,

day = "18",

doi = "10.1016/j.xpro.2022.101224",

language = "אנגלית",

volume = "3",

journal = "STAR Protocols",

issn = "2666-1667",

publisher = "Cell Press",

number = "1",

}

TY - JOUR

T1 - In vivo optical recordings of ion dynamics in mouse corneal primary nociceptive terminals

AU - Gershon, Devora

AU - Negev-Goldstein, Robert H.

AU - Abd al Razzaq, Lama

AU - Lev, Shaya

AU - Binshtok, Alexander M.

PY - 2022/3/18

Y1 - 2022/3/18

N2 - This protocol aims to measure ion dynamics in nociceptive terminal endings in intact mice in vivo. We describe viral injection of GCaMP6s + RFP into trigeminal ganglia (TG) of mice, followed by calcium imaging of corneal nociceptive terminals that express GCaMP6s and RFP. This fast and high-resolution optical recording technique enables studying a nociceptive terminal's functional molecular network in physiological and pathological conditions. This platform can be applied to studying the physiology of terminals of other neurons. For complete details on the use and execution of this protocol, please refer to Goldstein et al. (2019).

AB - This protocol aims to measure ion dynamics in nociceptive terminal endings in intact mice in vivo. We describe viral injection of GCaMP6s + RFP into trigeminal ganglia (TG) of mice, followed by calcium imaging of corneal nociceptive terminals that express GCaMP6s and RFP. This fast and high-resolution optical recording technique enables studying a nociceptive terminal's functional molecular network in physiological and pathological conditions. This platform can be applied to studying the physiology of terminals of other neurons. For complete details on the use and execution of this protocol, please refer to Goldstein et al. (2019).

KW - Microscopy

KW - Model Organisms

KW - Neuroscience

UR - http://www.scopus.com/inward/record.url?scp=85125629380&partnerID=8YFLogxK

U2 - 10.1016/j.xpro.2022.101224

DO - 10.1016/j.xpro.2022.101224

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 35284836

AN - SCOPUS:85125629380

SN - 2666-1667

VL - 3

JO - STAR Protocols

JF - STAR Protocols

IS - 1

M1 - 101224

ER -

In vivo optical recordings of ion dynamics in mouse corneal primary nociceptive terminals

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this