Abstract
The 'in planta' visualization of F-actin in all cells and in all developmental stages of a plant is a challenging problem. By using the soybean heat inducible Gmhsp17.3B promoter instead of a constitutive promoter, we have been able to label all cells in various developmental stages of the moss Physcomitrella patens, through a precise temperature tuning of the expression of green fluorescent protein (GFP)-talin. A short moderate heat treatment was sufficient to induce proper labeling of the actin cytoskeleton and to allow the visualization of time-dependent organization of F-actin structures without impairment of cell viability. In growing moss cells, dense converging arrays of F-actin structures were present at the growing tips of protonema cell, and at the localization of branching. Protonema and leaf cells contained a network of thick actin cables; during dedifferentiation of leaf cells into new protonema filaments, the thick bundled actin network disappeared, and a new highly polarized F-actin network formed. The controlled expression of GFP-talin through an inducible promoter improves significantly the 'in planta' imaging of actin.
Original language | English |
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Pages (from-to) | 63-76 |
Number of pages | 14 |
Journal | New Phytologist |
Volume | 174 |
Issue number | 1 |
DOIs | |
State | Published - Apr 2007 |
Externally published | Yes |
Keywords
- Actin microfilament
- ARP3
- Cell polarity
- Cytoskeleton
- Green fluorescent protein (GFP)-talin
- Heat-shock promoter
- Moss
- Physcomitrella patens