Inactivation of the extrinsic subunit of photosystem II, PsbU, in Synechococcus PCC 7942 results in elevated resistance to oxidative stress

Ido Balint; Jyotirmoy Bhattacharya; Alexander Perelman; Daniella Schatz; Yona Moskovitz; Nir Keren; Rakefet Schwarz

doi:10.1016/j.febslet.2006.03.020

Inactivation of the extrinsic subunit of photosystem II, PsbU, in Synechococcus PCC 7942 results in elevated resistance to oxidative stress

Ido Balint, Jyotirmoy Bhattacharya, Alexander Perelman, Daniella Schatz, Yona Moskovitz, Nir Keren, Rakefet Schwarz^*

^*Corresponding author for this work

Department of Plant and Environmental Sciences (Natural Sciences)

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

PsbU is a subunit of the extrinsic complex attached to the core of photosystem II. A PsbU-mutant of Synechococcus PCC 7942 was isolated based on its elevated resistance to externally applied oxidative stress. PsbU-mutant exhibits fast rates of degradation of the photosystem II core protein, D1, under sub-saturating as well as high-light conditions. While forward electron transfer is not affected, back electron flow is severely impaired in the mutant. We suggest that impairment of psbU results in production of reactive-oxygen-species, which trigger antioxidative mechanisms even under standard growth conditions. Accordingly, when challenged with external oxidative stress, these cells are more resistant than wild type cells.

Original language	English
Pages (from-to)	2117-2122
Number of pages	6
Journal	FEBS Letters
Volume	580
Issue number	8
DOIs	https://doi.org/10.1016/j.febslet.2006.03.020
State	Published - 3 Apr 2006

Bibliographical note

Funding Information:
This study was supported by the Israel Science Foundation (Grant No. 683/03-17.1) and by the German Israel Foundation (Grant No. I-729-27.9/2002). Work in the laboratory of N.K. is carried out under the auspices of the Avron Even-Ari Minerva Center.

Keywords

Cyanobacteria
D1 degradation
Oxidative stress
PsbU

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10.1016/j.febslet.2006.03.020

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title = "Inactivation of the extrinsic subunit of photosystem II, PsbU, in Synechococcus PCC 7942 results in elevated resistance to oxidative stress",

abstract = "PsbU is a subunit of the extrinsic complex attached to the core of photosystem II. A PsbU-mutant of Synechococcus PCC 7942 was isolated based on its elevated resistance to externally applied oxidative stress. PsbU-mutant exhibits fast rates of degradation of the photosystem II core protein, D1, under sub-saturating as well as high-light conditions. While forward electron transfer is not affected, back electron flow is severely impaired in the mutant. We suggest that impairment of psbU results in production of reactive-oxygen-species, which trigger antioxidative mechanisms even under standard growth conditions. Accordingly, when challenged with external oxidative stress, these cells are more resistant than wild type cells.",

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AU - Perelman, Alexander

AU - Schatz, Daniella

AU - Moskovitz, Yona

AU - Keren, Nir

AU - Schwarz, Rakefet

N1 - Funding Information: This study was supported by the Israel Science Foundation (Grant No. 683/03-17.1) and by the German Israel Foundation (Grant No. I-729-27.9/2002). Work in the laboratory of N.K. is carried out under the auspices of the Avron Even-Ari Minerva Center.

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N2 - PsbU is a subunit of the extrinsic complex attached to the core of photosystem II. A PsbU-mutant of Synechococcus PCC 7942 was isolated based on its elevated resistance to externally applied oxidative stress. PsbU-mutant exhibits fast rates of degradation of the photosystem II core protein, D1, under sub-saturating as well as high-light conditions. While forward electron transfer is not affected, back electron flow is severely impaired in the mutant. We suggest that impairment of psbU results in production of reactive-oxygen-species, which trigger antioxidative mechanisms even under standard growth conditions. Accordingly, when challenged with external oxidative stress, these cells are more resistant than wild type cells.

AB - PsbU is a subunit of the extrinsic complex attached to the core of photosystem II. A PsbU-mutant of Synechococcus PCC 7942 was isolated based on its elevated resistance to externally applied oxidative stress. PsbU-mutant exhibits fast rates of degradation of the photosystem II core protein, D1, under sub-saturating as well as high-light conditions. While forward electron transfer is not affected, back electron flow is severely impaired in the mutant. We suggest that impairment of psbU results in production of reactive-oxygen-species, which trigger antioxidative mechanisms even under standard growth conditions. Accordingly, when challenged with external oxidative stress, these cells are more resistant than wild type cells.

KW - Cyanobacteria

KW - D1 degradation

KW - Oxidative stress

KW - PsbU

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Inactivation of the extrinsic subunit of photosystem II, PsbU, in Synechococcus PCC 7942 results in elevated resistance to oxidative stress

Abstract

Bibliographical note

Keywords

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