Inactivation of the extrinsic subunit of photosystem II, PsbU, in Synechococcus PCC 7942 results in elevated resistance to oxidative stress

Ido Balint; Jyotirmoy Bhattacharya; Alexander Perelman; Daniella Schatz; Yona Moskovitz; Nir Keren; Rakefet Schwarz

doi:10.1016/j.febslet.2006.03.020

Inactivation of the extrinsic subunit of photosystem II, PsbU, in Synechococcus PCC 7942 results in elevated resistance to oxidative stress

Ido Balint
, Jyotirmoy Bhattacharya
, Alexander Perelman
, Daniella Schatz
, Yona Moskovitz
, Nir Keren
, Rakefet Schwarz^*

^*Corresponding author for this work

Department of Plant and Environmental Sciences (Natural Sciences)

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

PsbU is a subunit of the extrinsic complex attached to the core of photosystem II. A PsbU-mutant of Synechococcus PCC 7942 was isolated based on its elevated resistance to externally applied oxidative stress. PsbU-mutant exhibits fast rates of degradation of the photosystem II core protein, D1, under sub-saturating as well as high-light conditions. While forward electron transfer is not affected, back electron flow is severely impaired in the mutant. We suggest that impairment of psbU results in production of reactive-oxygen-species, which trigger antioxidative mechanisms even under standard growth conditions. Accordingly, when challenged with external oxidative stress, these cells are more resistant than wild type cells.

Original language	English
Pages (from-to)	2117-2122
Number of pages	6
Journal	FEBS Letters
Volume	580
Issue number	8
DOIs	https://doi.org/10.1016/j.febslet.2006.03.020
State	Published - 3 Apr 2006

Bibliographical note

Funding Information:
This study was supported by the Israel Science Foundation (Grant No. 683/03-17.1) and by the German Israel Foundation (Grant No. I-729-27.9/2002). Work in the laboratory of N.K. is carried out under the auspices of the Avron Even-Ari Minerva Center.

Keywords

Cyanobacteria
D1 degradation
Oxidative stress
PsbU

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10.1016/j.febslet.2006.03.020

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title = "Inactivation of the extrinsic subunit of photosystem II, PsbU, in Synechococcus PCC 7942 results in elevated resistance to oxidative stress",

abstract = "PsbU is a subunit of the extrinsic complex attached to the core of photosystem II. A PsbU-mutant of Synechococcus PCC 7942 was isolated based on its elevated resistance to externally applied oxidative stress. PsbU-mutant exhibits fast rates of degradation of the photosystem II core protein, D1, under sub-saturating as well as high-light conditions. While forward electron transfer is not affected, back electron flow is severely impaired in the mutant. We suggest that impairment of psbU results in production of reactive-oxygen-species, which trigger antioxidative mechanisms even under standard growth conditions. Accordingly, when challenged with external oxidative stress, these cells are more resistant than wild type cells.",

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note = "Funding Information: This study was supported by the Israel Science Foundation (Grant No. 683/03-17.1) and by the German Israel Foundation (Grant No. I-729-27.9/2002). Work in the laboratory of N.K. is carried out under the auspices of the Avron Even-Ari Minerva Center.",

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AU - Balint, Ido

AU - Bhattacharya, Jyotirmoy

AU - Perelman, Alexander

AU - Schatz, Daniella

AU - Moskovitz, Yona

AU - Keren, Nir

AU - Schwarz, Rakefet

N1 - Funding Information: This study was supported by the Israel Science Foundation (Grant No. 683/03-17.1) and by the German Israel Foundation (Grant No. I-729-27.9/2002). Work in the laboratory of N.K. is carried out under the auspices of the Avron Even-Ari Minerva Center.

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N2 - PsbU is a subunit of the extrinsic complex attached to the core of photosystem II. A PsbU-mutant of Synechococcus PCC 7942 was isolated based on its elevated resistance to externally applied oxidative stress. PsbU-mutant exhibits fast rates of degradation of the photosystem II core protein, D1, under sub-saturating as well as high-light conditions. While forward electron transfer is not affected, back electron flow is severely impaired in the mutant. We suggest that impairment of psbU results in production of reactive-oxygen-species, which trigger antioxidative mechanisms even under standard growth conditions. Accordingly, when challenged with external oxidative stress, these cells are more resistant than wild type cells.

AB - PsbU is a subunit of the extrinsic complex attached to the core of photosystem II. A PsbU-mutant of Synechococcus PCC 7942 was isolated based on its elevated resistance to externally applied oxidative stress. PsbU-mutant exhibits fast rates of degradation of the photosystem II core protein, D1, under sub-saturating as well as high-light conditions. While forward electron transfer is not affected, back electron flow is severely impaired in the mutant. We suggest that impairment of psbU results in production of reactive-oxygen-species, which trigger antioxidative mechanisms even under standard growth conditions. Accordingly, when challenged with external oxidative stress, these cells are more resistant than wild type cells.

KW - Cyanobacteria

KW - D1 degradation

KW - Oxidative stress

KW - PsbU

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JF - FEBS Letters

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Inactivation of the extrinsic subunit of photosystem II, PsbU, in Synechococcus PCC 7942 results in elevated resistance to oxidative stress

Abstract

Bibliographical note

Keywords

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