Increased humoral and cellular immunity in mice inoculated with allogeneic tumor cells attached to microcarrier beads

Eitan Yefenof; Steven Schwartz; Aviva Katz-Gross

doi:10.1016/0165-2478(83)90028-7

Increased humoral and cellular immunity in mice inoculated with allogeneic tumor cells attached to microcarrier beads

Eitan Yefenof^*, Steven Schwartz, Aviva Katz-Gross

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A murine carcinoma cell line was grown in a microcarrier culture and was used for immunization of allogeneic mice. It was found that inoculation of cells attached to microcarrier beads resulted in heightened serum titers of cytotoxic antibodies and in a stronger cell-mediated cytotoxic reactivity in the spleen compared to cells detached from the substrate. It is proposed that immunization of animals with anchorage-dependent cells should be carried out while the cells are still adherent to the culture microcarriers.

Original language	English
Pages (from-to)	39-43
Number of pages	5
Journal	Immunology Letters
Volume	6
Issue number	1
DOIs	https://doi.org/10.1016/0165-2478(83)90028-7
State	Published - Jan 1983

Keywords

adherent cell
immunization
microcarrier beads

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0165-2478(83)90028-7

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abstract = "A murine carcinoma cell line was grown in a microcarrier culture and was used for immunization of allogeneic mice. It was found that inoculation of cells attached to microcarrier beads resulted in heightened serum titers of cytotoxic antibodies and in a stronger cell-mediated cytotoxic reactivity in the spleen compared to cells detached from the substrate. It is proposed that immunization of animals with anchorage-dependent cells should be carried out while the cells are still adherent to the culture microcarriers.",

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AU - Schwartz, Steven

AU - Katz-Gross, Aviva

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N2 - A murine carcinoma cell line was grown in a microcarrier culture and was used for immunization of allogeneic mice. It was found that inoculation of cells attached to microcarrier beads resulted in heightened serum titers of cytotoxic antibodies and in a stronger cell-mediated cytotoxic reactivity in the spleen compared to cells detached from the substrate. It is proposed that immunization of animals with anchorage-dependent cells should be carried out while the cells are still adherent to the culture microcarriers.

AB - A murine carcinoma cell line was grown in a microcarrier culture and was used for immunization of allogeneic mice. It was found that inoculation of cells attached to microcarrier beads resulted in heightened serum titers of cytotoxic antibodies and in a stronger cell-mediated cytotoxic reactivity in the spleen compared to cells detached from the substrate. It is proposed that immunization of animals with anchorage-dependent cells should be carried out while the cells are still adherent to the culture microcarriers.

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KW - immunization

KW - microcarrier beads

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Increased humoral and cellular immunity in mice inoculated with allogeneic tumor cells attached to microcarrier beads

Abstract

Keywords

UN SDGs

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