Induced neuronal differentiation of human embryonic stem cells

Maya Schuldiner; Rachel Eiges; Amir Eden; Ofra Yanuka; Joseph Itskovitz-Eldor; Ronald S. Goldstein; Nissim Benvenisty

doi:10.1016/S0006-8993(01)02776-7

Induced neuronal differentiation of human embryonic stem cells

Maya Schuldiner, Rachel Eiges, Amir Eden, Ofra Yanuka, Joseph Itskovitz-Eldor, Ronald S. Goldstein, Nissim Benvenisty^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

370 Scopus citations

Abstract

Human embryonic stem (ES) cells are pluripotent cells capable of forming differentiated embryoid bodies (EBs) in culture. We examined the ability of growth factors under controlled conditions to increase the number of human ES cell-derived neurons. Retinoic acid (RA) and nerve growth factor (βNGF) were found to be potent enhancers of neuronal differentiation, eliciting extensive outgrowth of processes and the expression of neuron-specific molecules. Our findings show that human ES cells have great potential to become an unlimited cell source for neurons in culture. These cells may then be used in transplantation therapies for neural pathologies.

Original language	English
Pages (from-to)	201-205
Number of pages	5
Journal	Brain Research
Volume	913
Issue number	2
DOIs	https://doi.org/10.1016/S0006-8993(01)02776-7
State	Published - 21 Sep 2001

Bibliographical note

Funding Information:
This study was partially supported by the Juvenile Diabetes Foundation and by the Herbert Cohn Chair (N.B).

Keywords

Embryonic stem cell
Nerve growth factor
Neuronal differentiation
Retinoic acid
Transplantation therapy

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10.1016/S0006-8993(01)02776-7

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abstract = "Human embryonic stem (ES) cells are pluripotent cells capable of forming differentiated embryoid bodies (EBs) in culture. We examined the ability of growth factors under controlled conditions to increase the number of human ES cell-derived neurons. Retinoic acid (RA) and nerve growth factor (βNGF) were found to be potent enhancers of neuronal differentiation, eliciting extensive outgrowth of processes and the expression of neuron-specific molecules. Our findings show that human ES cells have great potential to become an unlimited cell source for neurons in culture. These cells may then be used in transplantation therapies for neural pathologies.",

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AU - Eiges, Rachel

AU - Eden, Amir

AU - Yanuka, Ofra

AU - Itskovitz-Eldor, Joseph

AU - Goldstein, Ronald S.

AU - Benvenisty, Nissim

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Induced neuronal differentiation of human embryonic stem cells

Abstract

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Keywords

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