Induction of autophagic flux by amino acid deprivation is distinct from nitrogen starvation-induced macroautophagy

Nitai Ecker, Angelica Mor, Dikla Journo, Hagai Abeliovich*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

A number of signaling mechanisms have been implicated in the regulation of autophagic trafficking. TOR kinase activity, cAMP levels, and the GAAC pathway have all been suggested to be involved. Here, we closely analyzed the stimuli that underlie induction of autophagic trafficking in Saccharomyces cerevisiae. We find evidence for the existence of a novel aspect of the autophagic pathway that is regulated by intracellular amino acids, uncoupled from extracellular nutrient levels, and is absolutely dependent on Gcn2 and Gcn4. This requirement for Gcn2 and Gcn4 distinguishes amino-acid starvation induced autophagy from classic macroautophagy: Macroautophagic flux in response to nitrogen starvation is only partly diminished in gcn2Δ and gcn4Δ cells. However this maintenance of autophagic flux in gcn mutants during nitrogen starvation reflects the formation of larger numbers of smaller autophagosomes. We report that gcn2Δ and gcn4Δ cells are defective in the induction of Atg8 and Atg4 upon starvation, and this defect results, during total nitrogen starvation, in the formation of abnormally small autophagosomes, although overall autophagic flux remains close to normal due to a compensatory increase in the overall number of autophagosomes.

Original languageAmerican English
Pages (from-to)879-890
Number of pages12
JournalAutophagy
Volume6
Issue number7
DOIs
StatePublished - 1 Oct 2010

Keywords

  • Atg8
  • Gcn2
  • Gcn4
  • Macroautophagy
  • Nitrogen starvation
  • Yeast

Fingerprint

Dive into the research topics of 'Induction of autophagic flux by amino acid deprivation is distinct from nitrogen starvation-induced macroautophagy'. Together they form a unique fingerprint.

Cite this