Induction of cell death in Arabidopsis by superoxide in combination with salicylic acid or with protein synthesis inhibitors

Alexander Mazel, Alex Levine*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Induction of programmed cell death (PCD) by oxidative stress is a widespread phenomenon in all living organisms. The degree of cell death depends on the concentration of oxidants and on environmental and physiological conditions. In plants, generation of reactive oxygen intermediates (ROI) occurs during many biotic and abiotic stresses. Recently, a number of spontaneous cell death mutants have been isolated in Arabidopsis. In one of the mutants (lsd1) induction of PCD has been attributed to superoxide (O2·-). Here we show that while in wild type plants generation of superoxide is symptomless, combination of O2·- with salicylic acid or with inhibitors of protein synthesis induced PCD. Cell death induced by these treatments was suppressed by protease inhibitors, indicating an active response. PCD induced by both treatments was preceded by nuclear condensation, which is a hallmark of apoptosis in plants and animals. These results may explain increased sensitivity to oxidative stress under certain physiological conditions, associated with high levels of salicylic acid or decrease in protein synthesis.

Original languageAmerican English
Pages (from-to)98-106
Number of pages9
JournalFree Radical Biology and Medicine
Volume30
Issue number1
DOIs
StatePublished - 1 Jan 2001

Bibliographical note

Funding Information:
This work was supported by the US-Israel Science Foundation (BSF). We thank Dr. J. Dangl for invaluable discussions.

Keywords

  • Arabidopsis
  • Cycloheximide
  • Free radicals
  • Programmed cell death
  • Salicylic acid
  • Superoxide

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