Induction of glucosyltransferase transcription and activity during superoxide-dependent cell death in Arabidopsis plants

Alexander Mazel; Alex Levine

doi:10.1016/S0981-9428(01)01351-1

Induction of glucosyltransferase transcription and activity during superoxide-dependent cell death in Arabidopsis plants

Alexander Mazel, Alex Levine^*

^*Corresponding author for this work

Department of Plant and Environmental Sciences (Natural Sciences)

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Expression of the UDP-glucosyltransferase (UGT73B5) gene is induced in Arabidopsis thaliana leaves during programmed cell death (PCD) stimulated by superoxide (O₂^.-) in the presence of cycloheximide (CHX) or salicylic acid (SA). Treatment with O₂^.-, CHX or SA alone did not result in PCD, and induced the UGT73B5 gene less strongly. Increased UDP-glucose:SA UGT activity was found in protein extracts from leaves treated with a combination of O₂^.- and SA. Genomic analysis showed that UGT73B5 is arranged in tandem with a similar UGT gene (UGT73B4) on chromosome 2. UGT73B5 promoter contains a G-box and several positive and negative SA-responsive elements. UGT73B5 transcription was also induced by mechanical cell crushing and by infection with an avirulent strain of Pseudomonas syringae or by treatment with intercellular fluid from Arabidopsis cell culture infected with a necrotrophic fungus, Botrytis cinerea. A possible role of UGT73B5 in the hypersensitive response is discussed.

Original language	American English
Pages (from-to)	133-140
Number of pages	8
Journal	Plant Physiology and Biochemistry
Volume	40
Issue number	2
DOIs	https://doi.org/10.1016/S0981-9428(01)01351-1
State	Published - 1 Feb 2002

Bibliographical note

Funding Information:
We thank Dr. J. Dangl (University of North Carolina) for invaluable discussions. We are grateful to Mazal Solomon for preparation of fungal elicitor from B. cinerea and to Ron Mittler for the rRNA probe. This work was supported by the US–Israel Science Foundation (BSF) and the Israel Academy of Sciences.

Keywords

Arabidopsis
Differential display
Glucosyltransferase
Hypersensitive response
Programmed cell death
Salicylic acid
Superoxide

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10.1016/S0981-9428(01)01351-1

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title = "Induction of glucosyltransferase transcription and activity during superoxide-dependent cell death in Arabidopsis plants",

abstract = "Expression of the UDP-glucosyltransferase (UGT73B5) gene is induced in Arabidopsis thaliana leaves during programmed cell death (PCD) stimulated by superoxide (O2.-) in the presence of cycloheximide (CHX) or salicylic acid (SA). Treatment with O2.-, CHX or SA alone did not result in PCD, and induced the UGT73B5 gene less strongly. Increased UDP-glucose:SA UGT activity was found in protein extracts from leaves treated with a combination of O2.- and SA. Genomic analysis showed that UGT73B5 is arranged in tandem with a similar UGT gene (UGT73B4) on chromosome 2. UGT73B5 promoter contains a G-box and several positive and negative SA-responsive elements. UGT73B5 transcription was also induced by mechanical cell crushing and by infection with an avirulent strain of Pseudomonas syringae or by treatment with intercellular fluid from Arabidopsis cell culture infected with a necrotrophic fungus, Botrytis cinerea. A possible role of UGT73B5 in the hypersensitive response is discussed.",

keywords = "Arabidopsis, Differential display, Glucosyltransferase, Hypersensitive response, Programmed cell death, Salicylic acid, Superoxide",

author = "Alexander Mazel and Alex Levine",

note = "Funding Information: We thank Dr. J. Dangl (University of North Carolina) for invaluable discussions. We are grateful to Mazal Solomon for preparation of fungal elicitor from B. cinerea and to Ron Mittler for the rRNA probe. This work was supported by the US–Israel Science Foundation (BSF) and the Israel Academy of Sciences.",

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AU - Levine, Alex

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N2 - Expression of the UDP-glucosyltransferase (UGT73B5) gene is induced in Arabidopsis thaliana leaves during programmed cell death (PCD) stimulated by superoxide (O2.-) in the presence of cycloheximide (CHX) or salicylic acid (SA). Treatment with O2.-, CHX or SA alone did not result in PCD, and induced the UGT73B5 gene less strongly. Increased UDP-glucose:SA UGT activity was found in protein extracts from leaves treated with a combination of O2.- and SA. Genomic analysis showed that UGT73B5 is arranged in tandem with a similar UGT gene (UGT73B4) on chromosome 2. UGT73B5 promoter contains a G-box and several positive and negative SA-responsive elements. UGT73B5 transcription was also induced by mechanical cell crushing and by infection with an avirulent strain of Pseudomonas syringae or by treatment with intercellular fluid from Arabidopsis cell culture infected with a necrotrophic fungus, Botrytis cinerea. A possible role of UGT73B5 in the hypersensitive response is discussed.

AB - Expression of the UDP-glucosyltransferase (UGT73B5) gene is induced in Arabidopsis thaliana leaves during programmed cell death (PCD) stimulated by superoxide (O2.-) in the presence of cycloheximide (CHX) or salicylic acid (SA). Treatment with O2.-, CHX or SA alone did not result in PCD, and induced the UGT73B5 gene less strongly. Increased UDP-glucose:SA UGT activity was found in protein extracts from leaves treated with a combination of O2.- and SA. Genomic analysis showed that UGT73B5 is arranged in tandem with a similar UGT gene (UGT73B4) on chromosome 2. UGT73B5 promoter contains a G-box and several positive and negative SA-responsive elements. UGT73B5 transcription was also induced by mechanical cell crushing and by infection with an avirulent strain of Pseudomonas syringae or by treatment with intercellular fluid from Arabidopsis cell culture infected with a necrotrophic fungus, Botrytis cinerea. A possible role of UGT73B5 in the hypersensitive response is discussed.

KW - Arabidopsis

KW - Differential display

KW - Glucosyltransferase

KW - Hypersensitive response

KW - Programmed cell death

KW - Salicylic acid

KW - Superoxide

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Induction of glucosyltransferase transcription and activity during superoxide-dependent cell death in Arabidopsis plants

Abstract

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Keywords

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