Abstract
We demonstrate that it is possible to reduce the induction time for formation of extracellular laccase by Botrytis cinerea. This was achieved by preincubating inductive medium with mycelium, followed by inoculation with fresh mycelium. We suggest that the active inducer is a product of gallic acid metabolism. Its formation can be prevented by trapping the quinone formed from gallic acid, using benzenesulphinic acid. We also show that induction of laccase formation can be inhibited by pre-treatment of the mycelium with 2,16,25-cucurbitacin D triacetate, prior to transfer to inductive medium. The implications for control of laccase formation are discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 47-49 |
| Number of pages | 3 |
| Journal | Phytochemistry |
| Volume | 34 |
| Issue number | 1 |
| DOIs | |
| State | Published - 3 Aug 1993 |
Keywords
- Botrytis cinerea
- Hyphomycetes
- cucurbitacin, gallic acid
- induction of enzyme formation.
- laccase