Infection studies of Pseudomonas Tomato, causal agent of Bacterial speck of Tomato

Y. Bashan, Y. Okon, Y. Henis*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Pseudomonas tomato, the causal agent of bacterial speck of tomato (Lycopersicon esculentum), was isolated on King B medium supplemented with penicillin and cycloheximide. An inoculum concentration of 104-105 colony-forming units per ml was used to obtain typical symptoms. Wounding of leaves by rubbing them with carborundum powder, or spraying with diluted wax solvent, significantly increased disease incidence. The best inoculation was obtained by subjecting the plants to 40°C for 30 min to 3 h or by incubating them for 24 h under mist prior to inoculation. The physiological age of the plants did not affect disease development. The bacteria survived in and were reisolated from soils, commercial seeds and plants after periods varying from 2 months to one year.

Original languageEnglish
Pages (from-to)135-143
Number of pages9
JournalPhytoparasitica
Volume6
Issue number3
DOIs
StatePublished - Oct 1978

Keywords

  • epidemiology
  • Lycopersicon esculentum

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