Abstract
Pseudomonas tomato, the causal agent of bacterial speck of tomato (Lycopersicon esculentum), was isolated on King B medium supplemented with penicillin and cycloheximide. An inoculum concentration of 104-105 colony-forming units per ml was used to obtain typical symptoms. Wounding of leaves by rubbing them with carborundum powder, or spraying with diluted wax solvent, significantly increased disease incidence. The best inoculation was obtained by subjecting the plants to 40°C for 30 min to 3 h or by incubating them for 24 h under mist prior to inoculation. The physiological age of the plants did not affect disease development. The bacteria survived in and were reisolated from soils, commercial seeds and plants after periods varying from 2 months to one year.
Original language | English |
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Pages (from-to) | 135-143 |
Number of pages | 9 |
Journal | Phytoparasitica |
Volume | 6 |
Issue number | 3 |
DOIs | |
State | Published - Oct 1978 |
Keywords
- epidemiology
- Lycopersicon esculentum