Influence of FAS on murine mast cell maturation

Beata Berent-Maoz, Chamutal Gur, Francesca Vita, Maria Rosa Soranzo, Giuliano Zabucchi, Francesca Levi-Schaffer*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Background: FAS has been shown to be involved in the regulation of many immune processes by induction of cellular apoptosis. However, accumulated evidence shows that FAS signaling also exhibits nonapoptotic functions, such as induction of cell proliferation and differentiation. FAS is the only death receptor known to be expressed on murine mast cells (MCs). Objective: To evaluate the role of FAS on murine MC maturation. Methods: Mouse bone marrow-derived MCs (BMMCs) or peritoneal MCs were derived from FAS-deficient, FASlpr/lpr, and congenic wild-type strains. The MC degranulation and cytokine release after IgE activation was assessed by measuring β-hexosaminidase, interleukin 13, and tumor necrosis factor α release. Transmission electron microscopy analysis was performed to evaluate the level of BMMC maturation. The surface markers and intracellular preformed mediators were measured as well. Results: Our data reveal that FAS deficiency has an impact on IgE-dependent activation of BMMCs, resulting in a significant decrease in β- hexosaminidase, interleukin 13, and tumor necrosis factor α release. The total content of preformed mediators (eg, tryptase and β-hexosaminidase) was reduced in BMMCs derived from FAS-deficient mice. We also found that the level of FcεRI in peritoneal mast cells from FAS-deficient mice was significantly diminished. FAS deficiency also influenced the kinetics of BMMC maturation as was revealed by transmission electron microscopy analysis. Conclusion: Our data show that FAS has an impact on the regulation of mouse MC maturation in vitro.

Original languageAmerican English
Pages (from-to)239-244
Number of pages6
JournalAnnals of Allergy, Asthma and Immunology
Issue number3
StatePublished - Mar 2011

Bibliographical note

Funding Information:
Funding Sources: This work was partly supported by a grant from the UK Aimwell Charitable Trust and by grant 3000002966 from the Chief Scientist's Office of the Ministry of Health in Israel to Dr Levi-Schaffer.


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