Infrared surface plasmon spectroscopy and biosensing

Victor Yashunsky*, Alexander Zilbershtein, Vladislav Lirtsman, Tal Marciano, Benjamin Aroeti, Michael Golosovsky, Dan Davidov

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

4 Scopus citations

Abstract

The cell morphology is a valuable indicator of the physical condition and general status of the cell. Here we demonstrate a methodology for noninvasive biosensing of adherent living cells. Our method is based on infrared reflection spectroscopy of living cells cultured on thin Au film. To characterize cell morphology we utilized the unique properties of the infrared surface plasmon (λ=1-3 μm) and infrared guided wave that travel inside the cell monolayer. We demonstrate that our method enables monitoring of submicron variations in cell morphology in real-time and in a labelfree manner. In addition to morphological characterization, our method allows investigation of chemical composition and molecular structure of cells through infrared absorption spectroscopy analysis.

Original languageEnglish
Title of host publicationPlasmonics in Biology and Medicine IX
DOIs
StatePublished - 2012
EventPlasmonics in Biology and Medicine IX - San Francisco, CA, United States
Duration: 22 Jan 201224 Jan 2012

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8234
ISSN (Print)1605-7422

Conference

ConferencePlasmonics in Biology and Medicine IX
Country/TerritoryUnited States
CitySan Francisco, CA
Period22/01/1224/01/12

Keywords

  • Biosensor
  • Evanescent Waves
  • Infrared spectroscopy
  • Label-free
  • Living Cells
  • Noninvasive method
  • Surface Plasmon

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