Infrared surface plasmon spectroscopy decodes early processes in epithelial host cells upon enteropathogenic Escherichia coli infection

Victor Yashunsky; Benjamin Aroeti

doi:10.1007/978-1-4939-2617-6_20

Infrared surface plasmon spectroscopy decodes early processes in epithelial host cells upon enteropathogenic Escherichia coli infection

Victor Yashunsky^*, Benjamin Aroeti

^*Corresponding author for this work

Department of Cell and Developmental Biology

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Enteropathogenic Escherichia coli (EPEC) is a generally noninvasive bacterial pathogen that causes diarrhea in humans. This microbe infects mainly the enterocytes of the small intestine. In this chapter we describe newly developed method, infrared surface plasmon resonance (IR-SPR) spectroscopy, for sensing pathogen infection of living cells. The IR-SPR method enables real-time and label-free monitoring of EPEC infection through highly sensitive measurement of the refractive index and height of the host epithelial cell monolayer. Our findings indicate the great potential of the IR-SPR tool to study the dynamics of host-pathogen interactions with high spatiotemporal sensitivity.

Original language	English
Title of host publication	Label-Free Biosensor Methods in Drug Discovery
Publisher	Springer New York
Pages	353-371
Number of pages	19
ISBN (Electronic)	9781493926176
ISBN (Print)	9781493926169
DOIs	https://doi.org/10.1007/978-1-4939-2617-6_20
State	Published - 6 Apr 2015

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015. All right reserved.

Keywords

EPEC
Epithelial host cells
Infrared
Spectroscopy
Surface plasmon resonance

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10.1007/978-1-4939-2617-6_20

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AB - Enteropathogenic Escherichia coli (EPEC) is a generally noninvasive bacterial pathogen that causes diarrhea in humans. This microbe infects mainly the enterocytes of the small intestine. In this chapter we describe newly developed method, infrared surface plasmon resonance (IR-SPR) spectroscopy, for sensing pathogen infection of living cells. The IR-SPR method enables real-time and label-free monitoring of EPEC infection through highly sensitive measurement of the refractive index and height of the host epithelial cell monolayer. Our findings indicate the great potential of the IR-SPR tool to study the dynamics of host-pathogen interactions with high spatiotemporal sensitivity.

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KW - Infrared

KW - Spectroscopy

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Infrared surface plasmon spectroscopy decodes early processes in epithelial host cells upon enteropathogenic Escherichia coli infection

Abstract

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