Infrared Surface Plasmon Spectroscopy Decodes Early Processes in Epithelial Host Cells upon Enteropathogenic Escherichia coli Infection

Yashunsky Victor, Benjamin Aroeti

Research output: Contribution to journalArticlepeer-review

Abstract

Enteropathogenic Escherichia coli (EPEC) is a generally noninvasive bacterial pathogen that causes diarrhea in humans. This microbe infects mainly the enterocytes of the small intestine. In this chapter we describe newly developed method, infrared surface plasmon resonance (IR-SPR) spectroscopy, for sensing pathogen infection of living cells. The IR-SPR method enables real-time and label-free monitoring of EPEC infection through highly sensitive measurement of the refractive index and height of the host epithelial cell monolayer. Our findings indicate the great potential of the IR-SPR tool to study the dynamics of host-pathogen interactions with high spatiotemporal sensitivity.

Original languageAmerican English
Pages (from-to)353-371
Number of pages19
JournalMethods in Pharmacology and Toxicology
Volume53
DOIs
StatePublished - 2015

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015.

Keywords

  • EPEC
  • Epithelial host cells
  • Infrared
  • Spectroscopy
  • Surface plasmon resonance

Fingerprint

Dive into the research topics of 'Infrared Surface Plasmon Spectroscopy Decodes Early Processes in Epithelial Host Cells upon Enteropathogenic Escherichia coli Infection'. Together they form a unique fingerprint.

Cite this