Infrared surface plasmon spectroscopy of living cells

V. Yashunsky; A. Zilberstein; T. Marciano; V. Lirtsman; M. Golosovsky; D. Davidov; B. Aroeti

doi:10.1063/1.3498133

Infrared surface plasmon spectroscopy of living cells

V. Yashunsky^*, A. Zilberstein, T. Marciano, V. Lirtsman, M. Golosovsky, D. Davidov, B. Aroeti

^*Corresponding author for this work

Department of Cell and Developmental Biology

Research output: Contribution to journal › Conference article › peer-review

6 Scopus citations

Abstract

We report a spectroscopic technique that combines the Fourier-Transform Infrared Spectroscopy (FTIR) with the Surface Plasmon Resonance (SPR). This FTIR-SPR technique enables sensitive infrared spectroscopy of liquid and solid objects in the attenuated total reflectance mode (ATR). The FTIR-SPR is similar to FTIR-ATR technique but has higher sensitivity due to resonance amplification of the surface electric field. FTIR-SPR is label-free method and it has unique advantages for studies of living cells. The FTIR-SPR also combines spectroscopic information inherent to FTIR with structural information provided by the Surface Plasmon Resonance (SPR). We discuss the FTIR-SPR technique for label-free studies of cell attachment to the substrate and for surface plasmon spectroscopy.

Original language	English
Pages (from-to)	1617-1621
Number of pages	5
Journal	AIP Conference Proceedings
Volume	1281
DOIs	https://doi.org/10.1063/1.3498133
State	Published - 2010
Event	International Conference on Numerical Analysis and Applied Mathematics 2010, ICNAAM-2010 - Rhodes, Greece Duration: 19 Sep 2010 → 25 Sep 2010

Keywords

FTIR
SPR
biosensor
cell culture
infrared
surface plasmon

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10.1063/1.3498133

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title = "Infrared surface plasmon spectroscopy of living cells",

abstract = "We report a spectroscopic technique that combines the Fourier-Transform Infrared Spectroscopy (FTIR) with the Surface Plasmon Resonance (SPR). This FTIR-SPR technique enables sensitive infrared spectroscopy of liquid and solid objects in the attenuated total reflectance mode (ATR). The FTIR-SPR is similar to FTIR-ATR technique but has higher sensitivity due to resonance amplification of the surface electric field. FTIR-SPR is label-free method and it has unique advantages for studies of living cells. The FTIR-SPR also combines spectroscopic information inherent to FTIR with structural information provided by the Surface Plasmon Resonance (SPR). We discuss the FTIR-SPR technique for label-free studies of cell attachment to the substrate and for surface plasmon spectroscopy.",

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author = "V. Yashunsky and A. Zilberstein and T. Marciano and V. Lirtsman and M. Golosovsky and D. Davidov and B. Aroeti",

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AU - Yashunsky, V.

AU - Zilberstein, A.

AU - Marciano, T.

AU - Lirtsman, V.

AU - Golosovsky, M.

AU - Davidov, D.

AU - Aroeti, B.

PY - 2010

Y1 - 2010

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AB - We report a spectroscopic technique that combines the Fourier-Transform Infrared Spectroscopy (FTIR) with the Surface Plasmon Resonance (SPR). This FTIR-SPR technique enables sensitive infrared spectroscopy of liquid and solid objects in the attenuated total reflectance mode (ATR). The FTIR-SPR is similar to FTIR-ATR technique but has higher sensitivity due to resonance amplification of the surface electric field. FTIR-SPR is label-free method and it has unique advantages for studies of living cells. The FTIR-SPR also combines spectroscopic information inherent to FTIR with structural information provided by the Surface Plasmon Resonance (SPR). We discuss the FTIR-SPR technique for label-free studies of cell attachment to the substrate and for surface plasmon spectroscopy.

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KW - SPR

KW - biosensor

KW - cell culture

KW - infrared

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Infrared surface plasmon spectroscopy of living cells

Abstract

Keywords

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