TY - JOUR
T1 - Inhibition of conjugation and cell division in Tetrahymena pyriformis by tunicamycin
T2 - A possible requirement of glycoprotein synthesis for induction of conjugation
AU - Frisch, A.
AU - Levkowitz, H.
AU - Loyter, A.
PY - 1976/9/7
Y1 - 1976/9/7
N2 - Tunicamycin, a glucosamine-containing antibiotic inhibited the conjugation process of Tetrahymena pyriformis. Sexual pairing was prevented completely when 1.5 μg/ml of tunicamycin was added to a mixture of the two mating types. Tunicamycin caused preferential inhibition of glycoprotein synthesis in Tetrahymena pyriformis. At 1.5 μg/ml and 6 μg/ml tunicamycin inhibited by 40% and 60% respectively [3H]-glucosamine incorporation into material precipitated by ethanol, while it did not affect [14C]-leucine incorporation. Cell division was also inhibited when the drug was added either to the regular growth medium or to the starvation medium.
AB - Tunicamycin, a glucosamine-containing antibiotic inhibited the conjugation process of Tetrahymena pyriformis. Sexual pairing was prevented completely when 1.5 μg/ml of tunicamycin was added to a mixture of the two mating types. Tunicamycin caused preferential inhibition of glycoprotein synthesis in Tetrahymena pyriformis. At 1.5 μg/ml and 6 μg/ml tunicamycin inhibited by 40% and 60% respectively [3H]-glucosamine incorporation into material precipitated by ethanol, while it did not affect [14C]-leucine incorporation. Cell division was also inhibited when the drug was added either to the regular growth medium or to the starvation medium.
UR - http://www.scopus.com/inward/record.url?scp=0017171567&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(76)90970-0
DO - 10.1016/0006-291X(76)90970-0
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C2 - 825115
AN - SCOPUS:0017171567
SN - 0006-291X
VL - 72
SP - 131
EP - 137
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -