Inhibition of dna metabolism in human b lymphocytes by a substrain of epstein-barr virus (P3hr-1): A method for virus quantitation

Michael Steinitz; Tibor Bakács; Eitan Yefenof; George Klein

doi:10.1159/000148977

Inhibition of dna metabolism in human b lymphocytes by a substrain of epstein-barr virus (P3hr-1): A method for virus quantitation

Michael Steinitz^*, Tibor Bakács, Eitan Yefenof, George Klein

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Pretreatment with the P3HR-1 substrain of Epstein-Barr virus (EBV) in-hibited approximately 85% of the DNA stimulation induced by Staphylococcus aureus in human B lymphocytes. In parallel experiments, the DNA stimulation induced by the trans-forming B95-8 substrain of EBV was almost completely inhibited by prior exposure to P3HR-1 virus. Phytohemagglutinin stimulation was only slightly inhibited, whereas the DNA synthesis of continuously growing Raji cells was inhibited to a considerable extent. The degree of DNA inhibition was correlated with the induction of the EBV-determined antigens (membrane, early, and nuclear antigens) in sensitive indicator cells.

Original language	English
Pages (from-to)	125-131
Number of pages	7
Journal	Intervirology
Volume	10
Issue number	2
DOIs	https://doi.org/10.1159/000148977
State	Published - 1978
Externally published	Yes

Keywords

B-cell DNA synthesis
Epstein-Barr virus
Inhibition
P3HR-I strain
Staphylococcus aureus sti-mulation

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10.1159/000148977

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title = "Inhibition of dna metabolism in human b lymphocytes by a substrain of epstein-barr virus (P3hr-1): A method for virus quantitation",

abstract = "Pretreatment with the P3HR-1 substrain of Epstein-Barr virus (EBV) in-hibited approximately 85% of the DNA stimulation induced by Staphylococcus aureus in human B lymphocytes. In parallel experiments, the DNA stimulation induced by the trans-forming B95-8 substrain of EBV was almost completely inhibited by prior exposure to P3HR-1 virus. Phytohemagglutinin stimulation was only slightly inhibited, whereas the DNA synthesis of continuously growing Raji cells was inhibited to a considerable extent. The degree of DNA inhibition was correlated with the induction of the EBV-determined antigens (membrane, early, and nuclear antigens) in sensitive indicator cells.",

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AU - Bakács, Tibor

AU - Yefenof, Eitan

AU - Klein, George

PY - 1978

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AB - Pretreatment with the P3HR-1 substrain of Epstein-Barr virus (EBV) in-hibited approximately 85% of the DNA stimulation induced by Staphylococcus aureus in human B lymphocytes. In parallel experiments, the DNA stimulation induced by the trans-forming B95-8 substrain of EBV was almost completely inhibited by prior exposure to P3HR-1 virus. Phytohemagglutinin stimulation was only slightly inhibited, whereas the DNA synthesis of continuously growing Raji cells was inhibited to a considerable extent. The degree of DNA inhibition was correlated with the induction of the EBV-determined antigens (membrane, early, and nuclear antigens) in sensitive indicator cells.

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Inhibition of dna metabolism in human b lymphocytes by a substrain of epstein-barr virus (P3hr-1): A method for virus quantitation

Abstract

Keywords

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