Inhibition of inorganic carbon transport by oxygen in a high CO2-requiring mutant (E1) of Anacystis nidulans R2

Teruo Ogawa*, Tatsuo Omata, Yehouda Marcus, Aaron Kaplan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The effect of O2 on inorganic carbon (Ci) transport was studied with a high CO2-requiring mutant (E1) of Anacystis nidulans R2. Oxygen (above 2%) inhibited Ci transport by 15-35|X% at CO2 concentrations above 200 μl/l, but had no apparent effect at low, limiting CO2 concentration. The action spectra for Ci transport measured in the presence or absence of 20% O2 showed two peaks around 684 and 625 nm, corresponding to chlorophyll a and phycocyanin absorption, respectively. The difference between these two spectra (anaerobic minus aerobic) showed one peak around 625 nm, which indicates that a linear electron transport from water to O2 is involved in the O2 inhibition of Ci transport. Dithiothreitol could overcome the inhibition by O2. The results suggested that the O2 inhibition is a result of inactivation of the Ci-transporting system.

Original languageEnglish
Pages (from-to)219-224
Number of pages6
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume893
Issue number2
DOIs
StatePublished - 10 Sep 1987

Keywords

  • (A. nidulans mutant)
  • Action spectrum
  • Carbon transport
  • Inorganic carbon transport
  • Oxygen inhibition

Fingerprint

Dive into the research topics of 'Inhibition of inorganic carbon transport by oxygen in a high CO2-requiring mutant (E1) of Anacystis nidulans R2'. Together they form a unique fingerprint.

Cite this