TY - JOUR
T1 - Inhibition of inorganic carbon transport by oxygen in a high CO2-requiring mutant (E1) of Anacystis nidulans R2
AU - Ogawa, Teruo
AU - Omata, Tatsuo
AU - Marcus, Yehouda
AU - Kaplan, Aaron
PY - 1987/9/10
Y1 - 1987/9/10
N2 - The effect of O2 on inorganic carbon (Ci) transport was studied with a high CO2-requiring mutant (E1) of Anacystis nidulans R2. Oxygen (above 2%) inhibited Ci transport by 15-35|X% at CO2 concentrations above 200 μl/l, but had no apparent effect at low, limiting CO2 concentration. The action spectra for Ci transport measured in the presence or absence of 20% O2 showed two peaks around 684 and 625 nm, corresponding to chlorophyll a and phycocyanin absorption, respectively. The difference between these two spectra (anaerobic minus aerobic) showed one peak around 625 nm, which indicates that a linear electron transport from water to O2 is involved in the O2 inhibition of Ci transport. Dithiothreitol could overcome the inhibition by O2. The results suggested that the O2 inhibition is a result of inactivation of the Ci-transporting system.
AB - The effect of O2 on inorganic carbon (Ci) transport was studied with a high CO2-requiring mutant (E1) of Anacystis nidulans R2. Oxygen (above 2%) inhibited Ci transport by 15-35|X% at CO2 concentrations above 200 μl/l, but had no apparent effect at low, limiting CO2 concentration. The action spectra for Ci transport measured in the presence or absence of 20% O2 showed two peaks around 684 and 625 nm, corresponding to chlorophyll a and phycocyanin absorption, respectively. The difference between these two spectra (anaerobic minus aerobic) showed one peak around 625 nm, which indicates that a linear electron transport from water to O2 is involved in the O2 inhibition of Ci transport. Dithiothreitol could overcome the inhibition by O2. The results suggested that the O2 inhibition is a result of inactivation of the Ci-transporting system.
KW - (A. nidulans mutant)
KW - Action spectrum
KW - Carbon transport
KW - Inorganic carbon transport
KW - Oxygen inhibition
UR - http://www.scopus.com/inward/record.url?scp=45949115194&partnerID=8YFLogxK
U2 - 10.1016/0005-2728(87)90042-9
DO - 10.1016/0005-2728(87)90042-9
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AN - SCOPUS:45949115194
SN - 0005-2728
VL - 893
SP - 219
EP - 224
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
IS - 2
ER -