Inhibition of inorganic carbon transport by oxygen in a high CO2-requiring mutant (E1) of Anacystis nidulans R2

Teruo Ogawa; Tatsuo Omata; Yehouda Marcus; Aaron Kaplan

doi:10.1016/0005-2728(87)90042-9

Inhibition of inorganic carbon transport by oxygen in a high CO₂-requiring mutant (E₁) of Anacystis nidulans R₂

Teruo Ogawa^*
, Tatsuo Omata
, Yehouda Marcus
, Aaron Kaplan

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The effect of O₂ on inorganic carbon (C_i) transport was studied with a high CO₂-requiring mutant (E₁) of Anacystis nidulans R₂. Oxygen (above 2%) inhibited C_i transport by 15-35|X% at CO₂ concentrations above 200 μl/l, but had no apparent effect at low, limiting CO₂ concentration. The action spectra for C_i transport measured in the presence or absence of 20% O₂ showed two peaks around 684 and 625 nm, corresponding to chlorophyll a and phycocyanin absorption, respectively. The difference between these two spectra (anaerobic minus aerobic) showed one peak around 625 nm, which indicates that a linear electron transport from water to O₂ is involved in the O₂ inhibition of C_i transport. Dithiothreitol could overcome the inhibition by O₂. The results suggested that the O₂ inhibition is a result of inactivation of the C_i-transporting system.

Original language	English
Pages (from-to)	219-224
Number of pages	6
Journal	Biochimica et Biophysica Acta - Bioenergetics
Volume	893
Issue number	2
DOIs	https://doi.org/10.1016/0005-2728(87)90042-9
State	Published - 10 Sep 1987

Keywords

(A. nidulans mutant)
Action spectrum
Carbon transport
Inorganic carbon transport
Oxygen inhibition

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10.1016/0005-2728(87)90042-9

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title = "Inhibition of inorganic carbon transport by oxygen in a high CO2-requiring mutant (E1) of Anacystis nidulans R2",

abstract = "The effect of O2 on inorganic carbon (Ci) transport was studied with a high CO2-requiring mutant (E1) of Anacystis nidulans R2. Oxygen (above 2\%) inhibited Ci transport by 15-35|X\% at CO2 concentrations above 200 μl/l, but had no apparent effect at low, limiting CO2 concentration. The action spectra for Ci transport measured in the presence or absence of 20\% O2 showed two peaks around 684 and 625 nm, corresponding to chlorophyll a and phycocyanin absorption, respectively. The difference between these two spectra (anaerobic minus aerobic) showed one peak around 625 nm, which indicates that a linear electron transport from water to O2 is involved in the O2 inhibition of Ci transport. Dithiothreitol could overcome the inhibition by O2. The results suggested that the O2 inhibition is a result of inactivation of the Ci-transporting system.",

keywords = "(A. nidulans mutant), Action spectrum, Carbon transport, Inorganic carbon transport, Oxygen inhibition",

author = "Teruo Ogawa and Tatsuo Omata and Yehouda Marcus and Aaron Kaplan",

year = "1987",

month = sep,

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doi = "10.1016/0005-2728(87)90042-9",

language = "אנגלית",

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AU - Ogawa, Teruo

AU - Omata, Tatsuo

AU - Marcus, Yehouda

AU - Kaplan, Aaron

PY - 1987/9/10

Y1 - 1987/9/10

N2 - The effect of O2 on inorganic carbon (Ci) transport was studied with a high CO2-requiring mutant (E1) of Anacystis nidulans R2. Oxygen (above 2%) inhibited Ci transport by 15-35|X% at CO2 concentrations above 200 μl/l, but had no apparent effect at low, limiting CO2 concentration. The action spectra for Ci transport measured in the presence or absence of 20% O2 showed two peaks around 684 and 625 nm, corresponding to chlorophyll a and phycocyanin absorption, respectively. The difference between these two spectra (anaerobic minus aerobic) showed one peak around 625 nm, which indicates that a linear electron transport from water to O2 is involved in the O2 inhibition of Ci transport. Dithiothreitol could overcome the inhibition by O2. The results suggested that the O2 inhibition is a result of inactivation of the Ci-transporting system.

AB - The effect of O2 on inorganic carbon (Ci) transport was studied with a high CO2-requiring mutant (E1) of Anacystis nidulans R2. Oxygen (above 2%) inhibited Ci transport by 15-35|X% at CO2 concentrations above 200 μl/l, but had no apparent effect at low, limiting CO2 concentration. The action spectra for Ci transport measured in the presence or absence of 20% O2 showed two peaks around 684 and 625 nm, corresponding to chlorophyll a and phycocyanin absorption, respectively. The difference between these two spectra (anaerobic minus aerobic) showed one peak around 625 nm, which indicates that a linear electron transport from water to O2 is involved in the O2 inhibition of Ci transport. Dithiothreitol could overcome the inhibition by O2. The results suggested that the O2 inhibition is a result of inactivation of the Ci-transporting system.

KW - (A. nidulans mutant)

KW - Action spectrum

KW - Carbon transport

KW - Inorganic carbon transport

KW - Oxygen inhibition

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JF - Biochimica et Biophysica Acta - Bioenergetics

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Inhibition of inorganic carbon transport by oxygen in a high CO₂-requiring mutant (E₁) of Anacystis nidulans R₂

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