Abstract
Chicken erythrocytes were fused either by Sendai virus or by the combination of Ca2+ and ionophore A23187. Intramembrane particles and external anionic sites of cells undergoing fusion were found to acquire the ability to undergo a process of cold-induced clustering (thermotropic separation). Cationized ferritin (200 μg/ml 5% (v/v) cell suspension) inhibited both the fusion process and the thermotropic separation of intramembrane particles and external anionic sites. The correlation between the mobility of membrane proteins and the fusion process is discussed. It is suggested that an increase in the lateral mobility of membrane proteins is a prerequisite for initiation of membrane fusion.
Original language | English |
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Pages (from-to) | 213-224 |
Number of pages | 12 |
Journal | Biochimica et Biophysica Acta - Biomembranes |
Volume | 514 |
Issue number | 2 |
DOIs | |
State | Published - 19 Dec 1978 |