Inhibition of replication of herpes simplex virus in mouse macrophages by interferons

I. Domke, P. Straub, H. Jacobsen, H. Kirchner, A. Panet

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

The replication of herpes simplex virus (HSV) type 1 in macrophages grown from spleen cells of mouse strains susceptible to HSV infection in vivo was very sensitive to interferon (IFN). Different types of mouse IFN (α, β, γ) exhibited similar antiviral activities. However, treatment of cells with IFN-γ in combination with IFN-α or IFN-β resulted in a synergistic inhibition of virus growth. As shown by assaying HSV DNA polymerase, IFN inhibited expression of the β-genes. Inhibition of enzyme induction correlated well with the reduction of viral yield. Induction of HSV DNA polymerase was delayed by IFN in a dose-dependent manner. These results show that IFN inhibits HSV replication at an early step prior to or during the synthesis of β-proteins.

Original languageEnglish
Pages (from-to)2231-2236
Number of pages6
JournalJournal of General Virology
Volume66
Issue number10
DOIs
StatePublished - 1985

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