Insight into plant cell wall chemistry and structure by combination of multiphoton microscopy with Raman imaging

Zsuzsanna Heiner; Ingrid Zeise; Rivka Elbaum; Janina Kneipp

doi:10.1002/jbio.201700164

Insight into plant cell wall chemistry and structure by combination of multiphoton microscopy with Raman imaging

Zsuzsanna Heiner, Ingrid Zeise, Rivka Elbaum, Janina Kneipp^*

^*Corresponding author for this work

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Spontaneous Raman scattering microspectroscopy, second harmonic generation (SHG) and 2-photon excited fluorescence (2PF) were used in combination to characterize the morphology together with the chemical composition of the cell wall in native plant tissues. As the data obtained with unstained sections of Sorghum bicolor root and leaf tissues illustrate, nonresonant as well as pre-resonant Raman microscopy in combination with hyperspectral analysis reveals details about the distribution and composition of the major cell wall constituents. Multivariate analysis of the Raman data allows separation of different tissue regions, specifically the endodermis, xylem and lumen. The orientation of cellulose microfibrils is obtained from polarization-resolved SHG signals. Furthermore, 2-photon autofluorescence images can be used to image lignification. The combined compositional, morphological and orientational information in the proposed coupling of SHG, Raman imaging and 2PF presents an extension of existing vibrational microspectroscopic imaging and multiphoton microscopic approaches not only for plant tissues.

Original language	American English
Article number	e201700164
Journal	Journal of Biophotonics
Volume	11
Issue number	4
DOIs	https://doi.org/10.1002/jbio.201700164
State	Published - Apr 2018

Bibliographical note

Funding Information:
We thank Harald Kneipp for valuable discussions and support in setting up experiments. We thank D. Lajkó and V. Tarabykin (Charité-Universitätsmedizin Berlin; Anatomy, Institute of Cell Biology and Neurobiology) for providing access to the vibratome and Peter Lasch for providing CytoSpec v.2.00.01. We thank Oshry Markovich (The Hebrew University of Jerusalem, Israel) for advice in cultivating plants. Financial support by ERC Starting Grant no. 259432 (MULTIBIOPHOT) to J.K., by DFG (GSC 1013 SALSA) to Z.H. and by Einstein Stiftung Berlin (grant A-2011-77, I.Z., J.K., R.E.) is gratefully acknowledged.

Publisher Copyright:
© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Keywords

2-photon autofluorescence
multivariate analysis
second harmonic imaging
sorghum
spontaneous Raman

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title = "Insight into plant cell wall chemistry and structure by combination of multiphoton microscopy with Raman imaging",

abstract = "Spontaneous Raman scattering microspectroscopy, second harmonic generation (SHG) and 2-photon excited fluorescence (2PF) were used in combination to characterize the morphology together with the chemical composition of the cell wall in native plant tissues. As the data obtained with unstained sections of Sorghum bicolor root and leaf tissues illustrate, nonresonant as well as pre-resonant Raman microscopy in combination with hyperspectral analysis reveals details about the distribution and composition of the major cell wall constituents. Multivariate analysis of the Raman data allows separation of different tissue regions, specifically the endodermis, xylem and lumen. The orientation of cellulose microfibrils is obtained from polarization-resolved SHG signals. Furthermore, 2-photon autofluorescence images can be used to image lignification. The combined compositional, morphological and orientational information in the proposed coupling of SHG, Raman imaging and 2PF presents an extension of existing vibrational microspectroscopic imaging and multiphoton microscopic approaches not only for plant tissues.",

keywords = "2-photon autofluorescence, multivariate analysis, second harmonic imaging, sorghum, spontaneous Raman",

author = "Zsuzsanna Heiner and Ingrid Zeise and Rivka Elbaum and Janina Kneipp",

note = "Funding Information: We thank Harald Kneipp for valuable discussions and support in setting up experiments. We thank D. Lajk{\'o} and V. Tarabykin (Charit{\'e}-Universit{\"a}tsmedizin Berlin; Anatomy, Institute of Cell Biology and Neurobiology) for providing access to the vibratome and Peter Lasch for providing CytoSpec v.2.00.01. We thank Oshry Markovich (The Hebrew University of Jerusalem, Israel) for advice in cultivating plants. Financial support by ERC Starting Grant no. 259432 (MULTIBIOPHOT) to J.K., by DFG (GSC 1013 SALSA) to Z.H. and by Einstein Stiftung Berlin (grant A-2011-77, I.Z., J.K., R.E.) is gratefully acknowledged. Publisher Copyright: {\textcopyright} 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",

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language = "American English",

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AU - Zeise, Ingrid

AU - Elbaum, Rivka

AU - Kneipp, Janina

N1 - Funding Information: We thank Harald Kneipp for valuable discussions and support in setting up experiments. We thank D. Lajkó and V. Tarabykin (Charité-Universitätsmedizin Berlin; Anatomy, Institute of Cell Biology and Neurobiology) for providing access to the vibratome and Peter Lasch for providing CytoSpec v.2.00.01. We thank Oshry Markovich (The Hebrew University of Jerusalem, Israel) for advice in cultivating plants. Financial support by ERC Starting Grant no. 259432 (MULTIBIOPHOT) to J.K., by DFG (GSC 1013 SALSA) to Z.H. and by Einstein Stiftung Berlin (grant A-2011-77, I.Z., J.K., R.E.) is gratefully acknowledged. Publisher Copyright: © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

PY - 2018/4

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N2 - Spontaneous Raman scattering microspectroscopy, second harmonic generation (SHG) and 2-photon excited fluorescence (2PF) were used in combination to characterize the morphology together with the chemical composition of the cell wall in native plant tissues. As the data obtained with unstained sections of Sorghum bicolor root and leaf tissues illustrate, nonresonant as well as pre-resonant Raman microscopy in combination with hyperspectral analysis reveals details about the distribution and composition of the major cell wall constituents. Multivariate analysis of the Raman data allows separation of different tissue regions, specifically the endodermis, xylem and lumen. The orientation of cellulose microfibrils is obtained from polarization-resolved SHG signals. Furthermore, 2-photon autofluorescence images can be used to image lignification. The combined compositional, morphological and orientational information in the proposed coupling of SHG, Raman imaging and 2PF presents an extension of existing vibrational microspectroscopic imaging and multiphoton microscopic approaches not only for plant tissues.

AB - Spontaneous Raman scattering microspectroscopy, second harmonic generation (SHG) and 2-photon excited fluorescence (2PF) were used in combination to characterize the morphology together with the chemical composition of the cell wall in native plant tissues. As the data obtained with unstained sections of Sorghum bicolor root and leaf tissues illustrate, nonresonant as well as pre-resonant Raman microscopy in combination with hyperspectral analysis reveals details about the distribution and composition of the major cell wall constituents. Multivariate analysis of the Raman data allows separation of different tissue regions, specifically the endodermis, xylem and lumen. The orientation of cellulose microfibrils is obtained from polarization-resolved SHG signals. Furthermore, 2-photon autofluorescence images can be used to image lignification. The combined compositional, morphological and orientational information in the proposed coupling of SHG, Raman imaging and 2PF presents an extension of existing vibrational microspectroscopic imaging and multiphoton microscopic approaches not only for plant tissues.

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Insight into plant cell wall chemistry and structure by combination of multiphoton microscopy with Raman imaging

Abstract

Bibliographical note

Keywords

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