Integrated oligoaniline-cross-linked composites of Au nanoparticles/ glucose oxidase electrodes: A generic paradigm for electrically contacted enzyme systems

Orner Yehezkeli, Yi Ming Yan, Ilina Baravik, Ran Tel-Vered, Itamar Willner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

69 Scopus citations

Abstract

Electropolymerizable glucose oxidase (GOx) was synthesized by the tethering of thioaniline units to the protein. The electropolymerization of the thioaniline-modified GOx in the presence of thioaniline-functionalized Au nanoparticles (NPs) yielded a three-dimensional bis-aniline-cross-linked Au NPs/GOx network. The bis-aniline bridging units are redox active and mediate the electron transfer between the enzyme redox sites and the electrode, and the Au NPs provide the three-dimensional conductivity for transporting the electrons to the electrode. The covalently linked GOx displays an effective electrical contact with the electrode (turnover rate of ca. 500 s-1, which results in a sensitive and selective glucose-sensing electrode. The preparation of the electrode by means of electropolymerization, and the bioelectrocatalytic features of the electrode, suggest its feasibility as a miniaturized electrode with a sensing matrix and as an implantable glucose sensor in future applications.

Original languageEnglish
Pages (from-to)2674-2679
Number of pages6
JournalChemistry - A European Journal
Volume15
Issue number11
DOIs
StatePublished - 2 Mar 2009

Keywords

  • Biosensors
  • Electropolymerization
  • Glucose oxidases
  • Gold
  • Nanostructures

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