Abstract
Electropolymerizable glucose oxidase (GOx) was synthesized by the tethering of thioaniline units to the protein. The electropolymerization of the thioaniline-modified GOx in the presence of thioaniline-functionalized Au nanoparticles (NPs) yielded a three-dimensional bis-aniline-cross-linked Au NPs/GOx network. The bis-aniline bridging units are redox active and mediate the electron transfer between the enzyme redox sites and the electrode, and the Au NPs provide the three-dimensional conductivity for transporting the electrons to the electrode. The covalently linked GOx displays an effective electrical contact with the electrode (turnover rate of ca. 500 s-1, which results in a sensitive and selective glucose-sensing electrode. The preparation of the electrode by means of electropolymerization, and the bioelectrocatalytic features of the electrode, suggest its feasibility as a miniaturized electrode with a sensing matrix and as an implantable glucose sensor in future applications.
Original language | English |
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Pages (from-to) | 2674-2679 |
Number of pages | 6 |
Journal | Chemistry - A European Journal |
Volume | 15 |
Issue number | 11 |
DOIs | |
State | Published - 2 Mar 2009 |
Keywords
- Biosensors
- Electropolymerization
- Glucose oxidases
- Gold
- Nanostructures