Integration of HIV-1 DNA is regulated by interplay between viral Rev and cellular LEDGF/p75 proteins

Aviad Levin, Joseph Rosenbluh, Zvi Hayouka, Assaf Friedler, Abraham Loyter*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

The present work describes a novel interaction between the human immunodeficiency virus type 1 (HIV-1) Rev protein and the cellular lens epithelium-derived growth factor p75 (LEDGF/p75) protein in vitro and in virus-infected cells. Here we show, for the first time, that formation of an Rev-LEDGF/p75 complex is a crucial step in regulating viral cDNA integration. Coimmunoprecipitation experiments at various times after virus infection revealed that, first, an integrase enzyme (IN)-LEDGF/p75 complex is formed, which is then replaced by a Rev-LEDGF/p75 and Rev-IN complexes. This was supported by in vitro experiments showing that Rev promotes dissociation of the IN-LEDGF/p75 complex. Combination of the viral IN and the cellular LEDGF/p75 is required for proper integration of the viral cDNA into the host chromosomal DNA. Our findings demonstrate that integration of HIV-1 cDNA is regulated by an interplay between viral Rev and the host-cell LEDGF/p75 proteins.

Original languageAmerican English
Pages (from-to)34-44
Number of pages11
JournalMolecular Medicine
Volume16
Issue number1-2
DOIs
StatePublished - Jan 2010

Bibliographical note

Funding Information:
This work was supported by the Israeli Science Foundation (AL) and by a start ing grant from the European Research Council (ERC) (to AF).

Fingerprint

Dive into the research topics of 'Integration of HIV-1 DNA is regulated by interplay between viral Rev and cellular LEDGF/p75 proteins'. Together they form a unique fingerprint.

Cite this