Integration of technologies for hepatic tissue engineering

Yaakov Nahmias, Francois Berthiaume, Martin L. Yarmush*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

137 Scopus citations

Abstract

The liver is the largest internal organ in the body, responsible for over 500 metabolic, regulatory, and immune functions. Loss of liver function leads to liver failure which causes over 25 000 deaths/year in the United States. Efforts in the field of hepatic tissue engineering include the design of bioartificial liver systems to prolong patient's lives during liver failure, for drug toxicity screening and for the study of liver regeneration, ischemia/reperfusion injury, fibrosis, viral infection, and inflammation. This chapter will overview the current state-of-the-art in hepatology including isolated perfused liver, culture of liver slices and tissue explants, hepatocyte culture on collagen "sandwich" and spheroids, coculture of hepatocytes with non-parenchymal cells, and the integration of these culture techniques with microfluidics and reactor design. This work will discuss the role of oxygen and medium composition in hepatocyte culture and present promising new technologies for hepatocyte proliferation and function. We will also discuss liver development, architecture, and function as they relate to these culture techniques. Finally, we will review current opportunities and major challenges in integrating cell culture, bioreactor design, and microtechnology to develop new systems for novel applications.

Original languageAmerican English
Title of host publicationTissue Engineering II
Subtitle of host publicationBasics of Tissue Engineering and Tissue Applications
EditorsKyongbum Lee, David Kaplan
Pages309-329
Number of pages21
DOIs
StatePublished - 2006
Externally publishedYes

Publication series

NameAdvances in Biochemical Engineering/Biotechnology
Volume103
ISSN (Print)0724-6145

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