Integrative structure and functional anatomy of a nuclear pore complex

Seung Joong Kim; Javier Fernandez-Martinez; Ilona Nudelman; Yi Shi; Wenzhu Zhang; Barak Raveh; Thurston Herricks; Brian D. Slaughter; Joanna A. Hogan; Paula Upla; Ilan E. Chemmama; Riccardo Pellarin; Ignacia Echeverria; Manjunatha Shivaraju; Azraa S. Chaudhury; Junjie Wang; Rosemary Williams; Jay R. Unruh; Charles H. Greenberg; Erica Y. Jacobs; Zhiheng Yu; M. Jason De La Cruz; Roxana Mironska; David L. Stokes; John D. Aitchison; Martin F. Jarrold; Jennifer L. Gerton; Steven J. Ludtke; Christopher W. Akey; Brian T. Chait; Andrej Sali; Michael P. Rout

doi:10.1038/nature26003

Integrative structure and functional anatomy of a nuclear pore complex

Seung Joong Kim, Javier Fernandez-Martinez, Ilona Nudelman, Yi Shi, Wenzhu Zhang, Barak Raveh, Thurston Herricks, Brian D. Slaughter, Joanna A. Hogan, Paula Upla, Ilan E. Chemmama, Riccardo Pellarin, Ignacia Echeverria, Manjunatha Shivaraju, Azraa S. Chaudhury, Junjie Wang, Rosemary Williams, Jay R. Unruh, Charles H. Greenberg, Erica Y. JacobsZhiheng Yu, M. Jason De La Cruz, Roxana Mironska, David L. Stokes, John D. Aitchison, Martin F. Jarrold, Jennifer L. Gerton, Steven J. Ludtke^*, Christopher W. Akey, Brian T. Chait, Andrej Sali, Michael P. Rout

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

347 Scopus citations

Abstract

Nuclear pore complexes play central roles as gatekeepers of RNA and protein transport between the cytoplasm and nucleoplasm. However, their large size and dynamic nature have impeded a full structural and functional elucidation. Here we determined the structure of the entire 552-protein nuclear pore complex of the yeast Saccharomyces cerevisiae at sub-nanometre precision by satisfying a wide range of data relating to the molecular arrangement of its constituents. The nuclear pore complex incorporates sturdy diagonal columns and connector cables attached to these columns, imbuing the structure with strength and flexibility. These cables also tie together all other elements of the nuclear pore complex, including membrane-interacting regions, outer rings and RNA-processing platforms. Inwardly directed anchors create a high density of transport factor-docking Phe-Gly repeats in the central channel, organized into distinct functional units. This integrative structure enables us to rationalize the architecture, transport mechanism and evolutionary origins of the nuclear pore complex.

Original language	English
Pages (from-to)	475-482
Number of pages	8
Journal	Nature
Volume	555
Issue number	7697
DOIs	https://doi.org/10.1038/nature26003
State	Published - 22 Mar 2018
Externally published	Yes

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© 2018 Macmillan Publishers Limited, part of Springer Nature. All rights reserved.

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Kim, S. J., Fernandez-Martinez, J., Nudelman, I., Shi, Y., Zhang, W., Raveh, B., Herricks, T., Slaughter, B. D., Hogan, J. A., Upla, P., Chemmama, I. E., Pellarin, R., Echeverria, I., Shivaraju, M., Chaudhury, A. S., Wang, J., Williams, R., Unruh, J. R., Greenberg, C. H., ... Rout, M. P. (2018). Integrative structure and functional anatomy of a nuclear pore complex. Nature, 555(7697), 475-482. https://doi.org/10.1038/nature26003

@article{5c57ac22e2bf49f394389373824613c2,

title = "Integrative structure and functional anatomy of a nuclear pore complex",

abstract = "Nuclear pore complexes play central roles as gatekeepers of RNA and protein transport between the cytoplasm and nucleoplasm. However, their large size and dynamic nature have impeded a full structural and functional elucidation. Here we determined the structure of the entire 552-protein nuclear pore complex of the yeast Saccharomyces cerevisiae at sub-nanometre precision by satisfying a wide range of data relating to the molecular arrangement of its constituents. The nuclear pore complex incorporates sturdy diagonal columns and connector cables attached to these columns, imbuing the structure with strength and flexibility. These cables also tie together all other elements of the nuclear pore complex, including membrane-interacting regions, outer rings and RNA-processing platforms. Inwardly directed anchors create a high density of transport factor-docking Phe-Gly repeats in the central channel, organized into distinct functional units. This integrative structure enables us to rationalize the architecture, transport mechanism and evolutionary origins of the nuclear pore complex.",

author = "Kim, {Seung Joong} and Javier Fernandez-Martinez and Ilona Nudelman and Yi Shi and Wenzhu Zhang and Barak Raveh and Thurston Herricks and Slaughter, {Brian D.} and Hogan, {Joanna A.} and Paula Upla and Chemmama, {Ilan E.} and Riccardo Pellarin and Ignacia Echeverria and Manjunatha Shivaraju and Chaudhury, {Azraa S.} and Junjie Wang and Rosemary Williams and Unruh, {Jay R.} and Greenberg, {Charles H.} and Jacobs, {Erica Y.} and Zhiheng Yu and {De La Cruz}, {M. Jason} and Roxana Mironska and Stokes, {David L.} and Aitchison, {John D.} and Jarrold, {Martin F.} and Gerton, {Jennifer L.} and Ludtke, {Steven J.} and Akey, {Christopher W.} and Chait, {Brian T.} and Andrej Sali and Rout, {Michael P.}",

year = "2018",

month = mar,

day = "22",

doi = "10.1038/nature26003",

language = "אנגלית",

volume = "555",

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journal = "Nature",

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Kim, SJ, Fernandez-Martinez, J, Nudelman, I, Shi, Y, Zhang, W, Raveh, B, Herricks, T, Slaughter, BD, Hogan, JA, Upla, P, Chemmama, IE, Pellarin, R, Echeverria, I, Shivaraju, M, Chaudhury, AS, Wang, J, Williams, R, Unruh, JR, Greenberg, CH, Jacobs, EY, Yu, Z, De La Cruz, MJ, Mironska, R, Stokes, DL, Aitchison, JD, Jarrold, MF, Gerton, JL, Ludtke, SJ, Akey, CW, Chait, BT, Sali, A & Rout, MP 2018, 'Integrative structure and functional anatomy of a nuclear pore complex', Nature, vol. 555, no. 7697, pp. 475-482. https://doi.org/10.1038/nature26003

TY - JOUR

T1 - Integrative structure and functional anatomy of a nuclear pore complex

AU - Kim, Seung Joong

AU - Fernandez-Martinez, Javier

AU - Nudelman, Ilona

AU - Shi, Yi

AU - Zhang, Wenzhu

AU - Raveh, Barak

AU - Herricks, Thurston

AU - Slaughter, Brian D.

AU - Hogan, Joanna A.

AU - Upla, Paula

AU - Chemmama, Ilan E.

AU - Pellarin, Riccardo

AU - Echeverria, Ignacia

AU - Shivaraju, Manjunatha

AU - Chaudhury, Azraa S.

AU - Wang, Junjie

AU - Williams, Rosemary

AU - Unruh, Jay R.

AU - Greenberg, Charles H.

AU - Jacobs, Erica Y.

AU - Yu, Zhiheng

AU - De La Cruz, M. Jason

AU - Mironska, Roxana

AU - Stokes, David L.

AU - Aitchison, John D.

AU - Jarrold, Martin F.

AU - Gerton, Jennifer L.

AU - Ludtke, Steven J.

AU - Akey, Christopher W.

AU - Chait, Brian T.

AU - Sali, Andrej

AU - Rout, Michael P.

PY - 2018/3/22

Y1 - 2018/3/22

N2 - Nuclear pore complexes play central roles as gatekeepers of RNA and protein transport between the cytoplasm and nucleoplasm. However, their large size and dynamic nature have impeded a full structural and functional elucidation. Here we determined the structure of the entire 552-protein nuclear pore complex of the yeast Saccharomyces cerevisiae at sub-nanometre precision by satisfying a wide range of data relating to the molecular arrangement of its constituents. The nuclear pore complex incorporates sturdy diagonal columns and connector cables attached to these columns, imbuing the structure with strength and flexibility. These cables also tie together all other elements of the nuclear pore complex, including membrane-interacting regions, outer rings and RNA-processing platforms. Inwardly directed anchors create a high density of transport factor-docking Phe-Gly repeats in the central channel, organized into distinct functional units. This integrative structure enables us to rationalize the architecture, transport mechanism and evolutionary origins of the nuclear pore complex.

AB - Nuclear pore complexes play central roles as gatekeepers of RNA and protein transport between the cytoplasm and nucleoplasm. However, their large size and dynamic nature have impeded a full structural and functional elucidation. Here we determined the structure of the entire 552-protein nuclear pore complex of the yeast Saccharomyces cerevisiae at sub-nanometre precision by satisfying a wide range of data relating to the molecular arrangement of its constituents. The nuclear pore complex incorporates sturdy diagonal columns and connector cables attached to these columns, imbuing the structure with strength and flexibility. These cables also tie together all other elements of the nuclear pore complex, including membrane-interacting regions, outer rings and RNA-processing platforms. Inwardly directed anchors create a high density of transport factor-docking Phe-Gly repeats in the central channel, organized into distinct functional units. This integrative structure enables us to rationalize the architecture, transport mechanism and evolutionary origins of the nuclear pore complex.

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DO - 10.1038/nature26003

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SN - 0028-0836

VL - 555

SP - 475

EP - 482

JO - Nature

JF - Nature

IS - 7697

ER -

Integrative structure and functional anatomy of a nuclear pore complex

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