Integrative structure modeling: Overview and assessment

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Abstract

Integrative structure modeling computationally combines data from multiple sources of information with the aim of obtaining structural insights that are not revealed by any single approach alone. In the first part of this review, we survey the commonly used sources of structural information and the computational aspects of model building. Throughout the past decade, integrative modeling was applied to various biological systems, with a focus on large protein complexes. Recent progress in the field of cryo-electron microscopy (cryo-EM) has resolved many of these complexes to near-atomic resolution. In the second part of this review, we compare a range of published integrative models with their higher-resolution counterparts with the aim of critically assessing their accuracy. This comparison gives a favorable view of integrative modeling and demonstrates its ability to yield accurate and informative results. We discuss possible roles of integrative modeling in the new era of cryo-EM and highlight future challenges and directions.

Original languageAmerican English
Pages (from-to)113-135
Number of pages23
JournalAnnual Review of Biochemistry
Volume88
DOIs
StatePublished - 20 Jun 2019

Bibliographical note

Publisher Copyright:
© 2019 by Annual Reviews. All rights reserved.

Keywords

  • cross-linking
  • cryo-EM
  • cryo-electron microscopy
  • integrative modeling
  • macromolecular assemblies
  • mass spectrometry
  • protein structure

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