Interaction between mast cells and glial cells: an in vitro study

Meir Shalit*, Talma Brenner, Esther Shohami, Francesca Levi-Schaffer

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Brain mast cells (MC) are located in close proximity to glial cells and it has been suggested that they belong to the connective tissue phenotype. To determine whether the local microenvironment provided by glial cells can influence mouse bone marrow-derived MC (BMMC), the putative counterpart of mucosal MC, we co-cultured these two cell types. BMMC numbers, morphology, histochemical properties and histamine content as well as glial cell morphology and function were evaluated up to 21 days. Our data indicate that BMMC adhere, proliferate, survive and can be activated to release histamine on the glial cell monolayers without changing their phenotype. Co-cultured glial cells preserve their morphological appearance and function throughout the culture period. These data indicate that central nervous system (CNS) glial cells do not induce phenotypic changes in BMMC and do not interfere with their viability and function.

Original languageAmerican English
Pages (from-to)195-199
Number of pages5
JournalJournal of Neuroimmunology
Issue number1-2
StatePublished - Mar 1993


  • Bone marrow-derived mast cells
  • Glial cells
  • Histamine
  • Thromboxane B


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