Interaction between the Saccharomyces cerevisiae CDC25 gene product and mammalian Ras

M. Segal, I. Marbach, D. Engelberg, G. Simchen, A. Levitzki*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


In order to characterize the interaction between the Saccharomyces cerevisiae Cdc25 protein and Harvey-ras (p21(H-ras)), we have constructed a yeast strain disrupted at the RAS1 and RAS2 loci, expressing both p21(H-ras) and the catalytic domain of the bovine GTPase activating protein (GAP) and containing the cdc25-2 mutation. Such a strain exhibits a temperature- sensitive phenotype. The shift to the nonpermissive temperature is accompanied by the loss of guanyl nucleotide-dependent activity of adenylylcyclase in vitro. The temperature-sensitive phenotype can be rescued by CDC25 itself, as well as by a plasmid containing a truncated SDC25 gene. In addition, wild type CDC25 significantly improves the guanyl nucleotide response observed in the background of the cdc25(ts) allele at the permissive temperature in a dosage-dependent manner and restores the guanyl nucleotide response at the restrictive temperature. Both CDC25 and a truncated SDC25 also restored p21(H-ras)-dependent guanyl nucleotide response in a strain isogenic to the one described above but containing a disrupted CDC25 locus instead of the temperature-sensitive allele. These results suggest that the S. cerevisiae Cdc25 protein interacts with p21(H-ras) expressed in yeast by promoting GDP-GTP exchange. It follows that the yeast system can be used for characterizing the interaction between guanyl nucleotide exchangers of Ras proteins and mammalian p21(H-ras).

Original languageAmerican English
Pages (from-to)22747-22751
Number of pages5
JournalJournal of Biological Chemistry
Issue number32
StatePublished - 1992


Dive into the research topics of 'Interaction between the Saccharomyces cerevisiae CDC25 gene product and mammalian Ras'. Together they form a unique fingerprint.

Cite this