Abstract
Strains of λ phage containing a mutated cII, cIII, or cy gene in addition to a cro- mutation were constructed. Each single mutant and the double mutants were analyzed for growth behavior and for expression of specific early and late λ proteins. λc1857cro 16 is unable to grow well at 30° and at 43°. This strain is incapable of turning off early genes (of the b region, of the PL and PR operons, and the cU and int genes) and is defective in the expression of late functions. The clear mutations permit phage growth at 30°. They relieve the inhibitory effect conferred by the cro- mutation on phage growth at 43° to different extents. Our results also indicate that the cII and cIII proteins do not necessarily act at y in the regulation of late functions.
Original language | English |
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Pages (from-to) | 426-436 |
Number of pages | 11 |
Journal | Virology |
Volume | 79 |
Issue number | 2 |
DOIs | |
State | Published - 15 Jun 1977 |