Interaction of cII, cIII, and cro gene products in the regulation of early and late functions of phage λ

Ariella Oppenheim, Marlene Belfort, Nurit Katzir, Nava Kass, Amos B. Oppenheim*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Strains of λ phage containing a mutated cII, cIII, or cy gene in addition to a cro- mutation were constructed. Each single mutant and the double mutants were analyzed for growth behavior and for expression of specific early and late λ proteins. λc1857cro 16 is unable to grow well at 30° and at 43°. This strain is incapable of turning off early genes (of the b region, of the PL and PR operons, and the cU and int genes) and is defective in the expression of late functions. The clear mutations permit phage growth at 30°. They relieve the inhibitory effect conferred by the cro- mutation on phage growth at 43° to different extents. Our results also indicate that the cII and cIII proteins do not necessarily act at y in the regulation of late functions.

Original languageEnglish
Pages (from-to)426-436
Number of pages11
JournalVirology
Volume79
Issue number2
DOIs
StatePublished - 15 Jun 1977

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