Interaction of FIE, a Polycomb protein, with pRb: A possible mechanism regulating endosperm development

A. Mosquna, A. Katz, S. Shochat, G. Grafi, N. Ohad*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Inactivation of the Arabidopsis protein FERTILIZATION INDEPENDENT ENDOSPERM (FIE) induces division of the central cell of the embryo sac, leading to endosperm development in the absence of fertilization. The mechanism whereby FIE regulates this process is unknown. We postulated that activation of central cell division in fie mutant plants might involve the retinoblastoma protein (pRb), a cell cycle regulatory element. Pull-down and surface plasmon resonance assays demonstrated that FIE interacts in-vitro with the pRb homologues from Arabidopsis (AtRb), maize (ZmRb) and human (HuRb). The interaction of FIE with ZmRB and HuRb in the yeast two-hybrid system supports the possibility that a FIE-pRb interaction may occur also in planta. Mutational analysis showed that this interaction does not occur via the LxCxE motif of the FIE protein nor via the pocket B domain of pRb. These results suggest that FIE may inhibit premature division of the central cell of the embryo sac, at least partly, through interaction with pRb, and suppression of pRb-regulated genes.

Original languageAmerican English
Pages (from-to)651-657
Number of pages7
JournalMolecular Genetics and Genomics
Volume271
Issue number6
DOIs
StatePublished - Jul 2004
Externally publishedYes

Bibliographical note

Funding Information:
Acknowledgements We acknowledge Drs. Leor Williams and Ofra Lotan for suggestions. This research was supported by grants awarded to N.O. by the Israel Sciences Foundation (Grant No. 503/00) and Grant No. IS-3158-99c from BARD, the USA-Israel Binational Agricultural Research and Development Fund

Keywords

  • Arabidopsis thaliana
  • Cell cycle regulation
  • Central cell of the embryo sac
  • Polycomb proteins
  • Retinoblastoma

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