TY - JOUR
T1 - Interaction of oxidized polyamines with DNA. II. Inactivation of transforming DNA
AU - Persky, S.
AU - Ephrati-Elizur, E.
AU - Bachrach, U.
PY - 1967/12/19
Y1 - 1967/12/19
N2 - 1. The effects of oxidized spermine on the biological activity of Bacillus subtilis DNA were studied by examining the transforming capacity of purified DNA treated in vitro, and of DNA spontaneously released from cells treated with oxidized spermine. 2. Transformation to tryptophan, histidine and methionine independence was affected significantly. The histidine marker was more sensitive to inactivation by oxidized spermine than the linked tryptophan and unlinked methionine markers. 3. NaCl, at high concentrations, antagonized the inhibitory effect of oxidized spermine, indicating the involvement of ionic factors in the binding of oxidized spermine to the DNA. 4. Transforming DNA after treatment with oxidized spermine, was more resistant to heating at 100° than untreated DNA, suggesting interstrand cross-linking.
AB - 1. The effects of oxidized spermine on the biological activity of Bacillus subtilis DNA were studied by examining the transforming capacity of purified DNA treated in vitro, and of DNA spontaneously released from cells treated with oxidized spermine. 2. Transformation to tryptophan, histidine and methionine independence was affected significantly. The histidine marker was more sensitive to inactivation by oxidized spermine than the linked tryptophan and unlinked methionine markers. 3. NaCl, at high concentrations, antagonized the inhibitory effect of oxidized spermine, indicating the involvement of ionic factors in the binding of oxidized spermine to the DNA. 4. Transforming DNA after treatment with oxidized spermine, was more resistant to heating at 100° than untreated DNA, suggesting interstrand cross-linking.
UR - http://www.scopus.com/inward/record.url?scp=0014211206&partnerID=8YFLogxK
U2 - 10.1016/0005-2787(67)90173-6
DO - 10.1016/0005-2787(67)90173-6
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C2 - 4966086
AN - SCOPUS:0014211206
SN - 0005-2787
VL - 149
SP - 459
EP - 466
JO - BBA Section Nucleic Acids And Protein Synthesis
JF - BBA Section Nucleic Acids And Protein Synthesis
IS - 2
ER -