Abstract
1. The effects of oxidized spermine on the biological activity of Bacillus subtilis DNA were studied by examining the transforming capacity of purified DNA treated in vitro, and of DNA spontaneously released from cells treated with oxidized spermine. 2. Transformation to tryptophan, histidine and methionine independence was affected significantly. The histidine marker was more sensitive to inactivation by oxidized spermine than the linked tryptophan and unlinked methionine markers. 3. NaCl, at high concentrations, antagonized the inhibitory effect of oxidized spermine, indicating the involvement of ionic factors in the binding of oxidized spermine to the DNA. 4. Transforming DNA after treatment with oxidized spermine, was more resistant to heating at 100° than untreated DNA, suggesting interstrand cross-linking.
| Original language | English |
|---|---|
| Pages (from-to) | 459-466 |
| Number of pages | 8 |
| Journal | BBA Section Nucleic Acids And Protein Synthesis |
| Volume | 149 |
| Issue number | 2 |
| DOIs | |
| State | Published - 19 Dec 1967 |
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