TY - JOUR
T1 - Interaction of oxidized polyamines with DNA. IV. Effect of DNA composition on the binding
AU - Bachrach, Uriel
AU - Eilon, Gabriel
PY - 1969/4/22
Y1 - 1969/4/22
N2 - 1. 1. Oxidized spermine, an iminodialdehyde obtained by the enzymic oxidation of spermine, is bound to DNA by electrostatic and covalent bonds. The covalent binding of oxidized spermine is irreversible and is completed after incubation with DNA for 30 h at 35°. 2. 2. The capacity of DNA to bind oxidized spermine is directly proportional to its guanine-cytosine content. This has been demonstrated by (a) alcohol precipitation of DNA-oxidized spermine complexes, (b) fractionation on hydroxyapatite columns, (c) alkali denaturation of complexes with DNA derived from various sources. 3. 3. Sonicated DNA has been fractionated on hydroxyapatite columns to yield guanine-cytosine-rich polynucleotides. These preparations have a higher capacity to bind oxidized spermine than polynucleotide chains poor in guanine-cytosine pairs. 4. 4. Native bihelical DNA binds 3-15 molecules of oxidized spermine per 103 nucleotide units. Denatured DNA has a higher binding capacity.
AB - 1. 1. Oxidized spermine, an iminodialdehyde obtained by the enzymic oxidation of spermine, is bound to DNA by electrostatic and covalent bonds. The covalent binding of oxidized spermine is irreversible and is completed after incubation with DNA for 30 h at 35°. 2. 2. The capacity of DNA to bind oxidized spermine is directly proportional to its guanine-cytosine content. This has been demonstrated by (a) alcohol precipitation of DNA-oxidized spermine complexes, (b) fractionation on hydroxyapatite columns, (c) alkali denaturation of complexes with DNA derived from various sources. 3. 3. Sonicated DNA has been fractionated on hydroxyapatite columns to yield guanine-cytosine-rich polynucleotides. These preparations have a higher capacity to bind oxidized spermine than polynucleotide chains poor in guanine-cytosine pairs. 4. 4. Native bihelical DNA binds 3-15 molecules of oxidized spermine per 103 nucleotide units. Denatured DNA has a higher binding capacity.
UR - http://www.scopus.com/inward/record.url?scp=0014687523&partnerID=8YFLogxK
U2 - 10.1016/0005-2787(69)90055-0
DO - 10.1016/0005-2787(69)90055-0
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C2 - 4890602
AN - SCOPUS:0014687523
SN - 0005-2787
VL - 179
SP - 473
EP - 483
JO - BBA Section Nucleic Acids And Protein Synthesis
JF - BBA Section Nucleic Acids And Protein Synthesis
IS - 2
ER -