TY - JOUR
T1 - Interaction of reconstituted Sendai viral envelopes with sperm cells
T2 - reconstituted Sendai virus envelope-induced fusion-mediated introduction of foreign material into bull sperm cells
AU - Nussbaum, O.
AU - Loyter, A.
PY - 1995/9
Y1 - 1995/9
N2 - Reconstituted Sendai virus envelopes (RSVE), i.e. membrane vesicles bearing the viral envelope glycoproteins and phospholipids, are able to fuse with bull sperm cells. This was inferred from the increase in the degree of fluorescence dequenching (DQ) obtained following incubation of fluorescently labeled (R18 labeled) RSVE with bull sperm cells and from electron microscopy studies of RSVE-sperm interaction. Only a low degree of DQ was observed, under the same conditions, with non-fusogenic fluorescently labeled RSVE. This, and electron microscopy results, show that binding and membrane fusion events occur between RSVE and sperm cells. In addition, DQ was observed following incubation of RSVE that had been pre-loaded with the self-quenched fluorochrome Calcein, with bull sperm cells, indicating fusion-mediated injection of the dye from the RSVE space into the sperm cells.
AB - Reconstituted Sendai virus envelopes (RSVE), i.e. membrane vesicles bearing the viral envelope glycoproteins and phospholipids, are able to fuse with bull sperm cells. This was inferred from the increase in the degree of fluorescence dequenching (DQ) obtained following incubation of fluorescently labeled (R18 labeled) RSVE with bull sperm cells and from electron microscopy studies of RSVE-sperm interaction. Only a low degree of DQ was observed, under the same conditions, with non-fusogenic fluorescently labeled RSVE. This, and electron microscopy results, show that binding and membrane fusion events occur between RSVE and sperm cells. In addition, DQ was observed following incubation of RSVE that had been pre-loaded with the self-quenched fluorochrome Calcein, with bull sperm cells, indicating fusion-mediated injection of the dye from the RSVE space into the sperm cells.
UR - http://www.scopus.com/inward/record.url?scp=0028794833&partnerID=8YFLogxK
U2 - 10.1007/BF01322534
DO - 10.1007/BF01322534
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C2 - 7487492
AN - SCOPUS:0028794833
SN - 0304-8608
VL - 140
SP - 1613
EP - 1622
JO - Archives of Virology
JF - Archives of Virology
IS - 9
ER -