TY - JOUR
T1 - Interaction of the β‐adrenergic receptor with Gs following delipidation
T2 - Specific lipid requirements for Gs activation and GTPase function
AU - BEN‐ARIE, Nissim
AU - GILEADI, Carina
AU - SCHRAMM, Michael
PY - 1988/10
Y1 - 1988/10
N2 - Preparations of β‐adrenergic receptor and Gs from turkey erythrocytes were delipidated by previously developed procedures. Three synthetic phospholipids, dioleoylglycerophosphoethanolamine, dioleoylglycerophosphocholine and dioleoylglycerophosphoserine plus an unphosphorylated lipid, were all required to restore receptor‐mediated activation of Gs by GTP[γS]. The same lipids were necessary for the reconstitution of the isoproterenol‐enhanced GTPase. The requirement for the unphosphorylated lipid could be fulfilled by 1‐monooleoyl glycerol, α‐tocopherol or oleic acid.
AB - Preparations of β‐adrenergic receptor and Gs from turkey erythrocytes were delipidated by previously developed procedures. Three synthetic phospholipids, dioleoylglycerophosphoethanolamine, dioleoylglycerophosphocholine and dioleoylglycerophosphoserine plus an unphosphorylated lipid, were all required to restore receptor‐mediated activation of Gs by GTP[γS]. The same lipids were necessary for the reconstitution of the isoproterenol‐enhanced GTPase. The requirement for the unphosphorylated lipid could be fulfilled by 1‐monooleoyl glycerol, α‐tocopherol or oleic acid.
UR - http://www.scopus.com/inward/record.url?scp=0023804965&partnerID=8YFLogxK
U2 - 10.1111/j.1432-1033.1988.tb14326.x
DO - 10.1111/j.1432-1033.1988.tb14326.x
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 2844534
AN - SCOPUS:0023804965
SN - 0014-2956
VL - 176
SP - 649
EP - 654
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 3
ER -