Interaction of the β‐adrenergic receptor with Gs following delipidation: Specific lipid requirements for Gs activation and GTPase function

Nissim BEN‐ARIE, Carina GILEADI, Michael SCHRAMM*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Preparations of β‐adrenergic receptor and Gs from turkey erythrocytes were delipidated by previously developed procedures. Three synthetic phospholipids, dioleoylglycerophosphoethanolamine, dioleoylglycerophosphocholine and dioleoylglycerophosphoserine plus an unphosphorylated lipid, were all required to restore receptor‐mediated activation of Gs by GTP[γS]. The same lipids were necessary for the reconstitution of the isoproterenol‐enhanced GTPase. The requirement for the unphosphorylated lipid could be fulfilled by 1‐monooleoyl glycerol, α‐tocopherol or oleic acid.

Original languageAmerican English
Pages (from-to)649-654
Number of pages6
JournalEuropean Journal of Biochemistry
Volume176
Issue number3
DOIs
StatePublished - Oct 1988

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