Preparations of β‐adrenergic receptor and Gs from turkey erythrocytes were delipidated by previously developed procedures. Three synthetic phospholipids, dioleoylglycerophosphoethanolamine, dioleoylglycerophosphocholine and dioleoylglycerophosphoserine plus an unphosphorylated lipid, were all required to restore receptor‐mediated activation of Gs by GTP[γS]. The same lipids were necessary for the reconstitution of the isoproterenol‐enhanced GTPase. The requirement for the unphosphorylated lipid could be fulfilled by 1‐monooleoyl glycerol, α‐tocopherol or oleic acid.
|Original language||American English|
|Number of pages||6|
|Journal||European Journal of Biochemistry|
|State||Published - Oct 1988|