Interactions of La3+ with phosphatidylserine vesicles binding, phase transition, leakage and fusion

M. M. Hammoudah; S. Nir; T. Isac; R. Kornhauser; T. P. Stewart; S. W. Hui; W. L.C. Vaz

doi:10.1016/0005-2736(79)90270-0

Interactions of La³⁺ with phosphatidylserine vesicles binding, phase transition, leakage and fusion

M. M. Hammoudah
, S. Nir^*
, T. Isac
, R. Kornhauser
, T. P. Stewart
, S. W. Hui
, W. L.C. Vaz

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

The interaction of La³⁺ with phosphatidylserine vesicles is elucidated by binding studies, differential scanning calorimetry, X-ray diffraction, freeze fracture electron microscopy, and release of vesicle contents. La³⁺ effectively competes with Ca²⁺ for phosphatidylserine binding sites. The saturation level is close to a La/lipid ratio of 1:3. A concentration of 0.1 mM of La³⁺ is sufficient to induce fusion between sonicated vesicles.

Original language	English
Pages (from-to)	338-343
Number of pages	6
Journal	Biochimica et Biophysica Acta - Biomembranes
Volume	558
Issue number	3
DOIs	https://doi.org/10.1016/0005-2736(79)90270-0
State	Published - 12 Dec 1979
Externally published	Yes

Keywords

(X-ray diffraction, Electron microscopy, Differential scanning calorimetry)
La binding
Membrane fusion
Phase transition

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10.1016/0005-2736(79)90270-0

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title = "Interactions of La3+ with phosphatidylserine vesicles binding, phase transition, leakage and fusion",

abstract = "The interaction of La3+ with phosphatidylserine vesicles is elucidated by binding studies, differential scanning calorimetry, X-ray diffraction, freeze fracture electron microscopy, and release of vesicle contents. La3+ effectively competes with Ca2+ for phosphatidylserine binding sites. The saturation level is close to a La/lipid ratio of 1:3. A concentration of 0.1 mM of La3+ is sufficient to induce fusion between sonicated vesicles.",

keywords = "(X-ray diffraction, Electron microscopy, Differential scanning calorimetry), La binding, Membrane fusion, Phase transition",

author = "Hammoudah, \{M. M.\} and S. Nir and T. Isac and R. Kornhauser and Stewart, \{T. P.\} and Hui, \{S. W.\} and Vaz, \{W. L.C.\}",

year = "1979",

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doi = "10.1016/0005-2736(79)90270-0",

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T1 - Interactions of La3+ with phosphatidylserine vesicles binding, phase transition, leakage and fusion

AU - Hammoudah, M. M.

AU - Nir, S.

AU - Isac, T.

AU - Kornhauser, R.

AU - Stewart, T. P.

AU - Hui, S. W.

AU - Vaz, W. L.C.

PY - 1979/12/12

Y1 - 1979/12/12

N2 - The interaction of La3+ with phosphatidylserine vesicles is elucidated by binding studies, differential scanning calorimetry, X-ray diffraction, freeze fracture electron microscopy, and release of vesicle contents. La3+ effectively competes with Ca2+ for phosphatidylserine binding sites. The saturation level is close to a La/lipid ratio of 1:3. A concentration of 0.1 mM of La3+ is sufficient to induce fusion between sonicated vesicles.

AB - The interaction of La3+ with phosphatidylserine vesicles is elucidated by binding studies, differential scanning calorimetry, X-ray diffraction, freeze fracture electron microscopy, and release of vesicle contents. La3+ effectively competes with Ca2+ for phosphatidylserine binding sites. The saturation level is close to a La/lipid ratio of 1:3. A concentration of 0.1 mM of La3+ is sufficient to induce fusion between sonicated vesicles.

KW - (X-ray diffraction, Electron microscopy, Differential scanning calorimetry)

KW - La binding

KW - Membrane fusion

KW - Phase transition

UR - https://www.scopus.com/pages/publications/0018789087

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JO - Biochimica et Biophysica Acta - Biomembranes

JF - Biochimica et Biophysica Acta - Biomembranes

IS - 3

ER -

Interactions of La³⁺ with phosphatidylserine vesicles binding, phase transition, leakage and fusion

Abstract

Keywords

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