TY - JOUR
T1 - Interactions of Microorganisms with Lipid Langmuir Layers
AU - Leader, Avia
AU - Molad, Ori
AU - Dombrovsky, Aviv
AU - Reches, Meital
AU - Mandler, Daniel
N1 - Publisher Copyright:
© 2021 American Chemical Society.
PY - 2021/8/31
Y1 - 2021/8/31
N2 - Preventing microbial contamination of aquatic environments is crucial for the proper supply of drinking water. Hence, understanding the interactions that govern bacterial and virus adsorption to surfaces is crucial to prevent infection transmittance. Here, we describe a new approach for studying the organization and interactions of various microorganisms, namely, Escherichia coli (E. coli) bacteria, E. coli-specific bacteriophage T4, and plant cucumber green mottle mosaic viruses (CGMMV), at the air/water interface using the Langmuir-Blodgett (LB) technique. CGMMV were found as applicable candidates for further studying their interactions with Langmuir lipid monolayers. The zwitterionic, positively, and negatively charged LB lipid monolayers with adsorbed viruses were deposited onto solid supports and characterized by atomic force microscopy. Using polymerase chain reaction, we indicated that the adsorption of CGMMV onto the LB monolayer is a result of electrostatic interactions. These insights are useful in engineering membrane filters that prevent biofouling for efficient purification systems.
AB - Preventing microbial contamination of aquatic environments is crucial for the proper supply of drinking water. Hence, understanding the interactions that govern bacterial and virus adsorption to surfaces is crucial to prevent infection transmittance. Here, we describe a new approach for studying the organization and interactions of various microorganisms, namely, Escherichia coli (E. coli) bacteria, E. coli-specific bacteriophage T4, and plant cucumber green mottle mosaic viruses (CGMMV), at the air/water interface using the Langmuir-Blodgett (LB) technique. CGMMV were found as applicable candidates for further studying their interactions with Langmuir lipid monolayers. The zwitterionic, positively, and negatively charged LB lipid monolayers with adsorbed viruses were deposited onto solid supports and characterized by atomic force microscopy. Using polymerase chain reaction, we indicated that the adsorption of CGMMV onto the LB monolayer is a result of electrostatic interactions. These insights are useful in engineering membrane filters that prevent biofouling for efficient purification systems.
UR - http://www.scopus.com/inward/record.url?scp=85114163949&partnerID=8YFLogxK
U2 - 10.1021/acs.langmuir.1c01431
DO - 10.1021/acs.langmuir.1c01431
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C2 - 34461726
AN - SCOPUS:85114163949
SN - 0743-7463
VL - 37
SP - 10340
EP - 10347
JO - Langmuir
JF - Langmuir
IS - 34
ER -