TY - JOUR
T1 - Interactions of sendai virus with plant protoplasts
AU - Salts, Yehiam
AU - Beckmann, Jacques S.
AU - Loyter, Abraham
AU - Lavi, Uri
PY - 1985/10/25
Y1 - 1985/10/25
N2 - Sendai virus (SV), a fusogenic agent in animal cells, was investigated for its potential to interact and possibly fuse with the plasma membranes of plant protoplasts. Adsorption of fluorescently-labelled viral particles was observed to be dependent on the presence of concanavalin A (ConA). This interaction could not be reproduced by wheat germ agglutinin. ConA adsorption to protoplasts could be reversed by an excess of free competing sugar moieties (α-methyl-mannoside, αMM) at any time. Complexes between Sendai virions, ConA and protoplasts could be dissociated by αMM, only shortly after their formation. Prolonged incubation of these complexes prevented their dissociation upon the later addition of the competing sugar. Neither dithiothreitol nor trypsinization of the viruses, known to affect the viral fusogenic ability to animal cells, modified the described adsorption properties. This is taken to mean that irreversible binding of Sendai viral particles to protoplast plasma membranes does occur, presumably via a different mechanism from that with animal cells. This should encourage studies aimed at testing the capacity of the reconstituted Sendai envelopes to serve as delivery carriers in plant genetic engineering projects.
AB - Sendai virus (SV), a fusogenic agent in animal cells, was investigated for its potential to interact and possibly fuse with the plasma membranes of plant protoplasts. Adsorption of fluorescently-labelled viral particles was observed to be dependent on the presence of concanavalin A (ConA). This interaction could not be reproduced by wheat germ agglutinin. ConA adsorption to protoplasts could be reversed by an excess of free competing sugar moieties (α-methyl-mannoside, αMM) at any time. Complexes between Sendai virions, ConA and protoplasts could be dissociated by αMM, only shortly after their formation. Prolonged incubation of these complexes prevented their dissociation upon the later addition of the competing sugar. Neither dithiothreitol nor trypsinization of the viruses, known to affect the viral fusogenic ability to animal cells, modified the described adsorption properties. This is taken to mean that irreversible binding of Sendai viral particles to protoplast plasma membranes does occur, presumably via a different mechanism from that with animal cells. This should encourage studies aimed at testing the capacity of the reconstituted Sendai envelopes to serve as delivery carriers in plant genetic engineering projects.
KW - concanavalin A
KW - lectins
KW - protoplast membranes
KW - transformations
UR - http://www.scopus.com/inward/record.url?scp=46549092815&partnerID=8YFLogxK
U2 - 10.1016/0168-9452(85)90116-5
DO - 10.1016/0168-9452(85)90116-5
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AN - SCOPUS:46549092815
SN - 0168-9452
VL - 41
SP - 141
EP - 149
JO - Plant Science
JF - Plant Science
IS - 2
ER -