Interactions of sendai virus with plant protoplasts

Yehiam Salts*, Jacques S. Beckmann, Abraham Loyter, Uri Lavi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Sendai virus (SV), a fusogenic agent in animal cells, was investigated for its potential to interact and possibly fuse with the plasma membranes of plant protoplasts. Adsorption of fluorescently-labelled viral particles was observed to be dependent on the presence of concanavalin A (ConA). This interaction could not be reproduced by wheat germ agglutinin. ConA adsorption to protoplasts could be reversed by an excess of free competing sugar moieties (α-methyl-mannoside, αMM) at any time. Complexes between Sendai virions, ConA and protoplasts could be dissociated by αMM, only shortly after their formation. Prolonged incubation of these complexes prevented their dissociation upon the later addition of the competing sugar. Neither dithiothreitol nor trypsinization of the viruses, known to affect the viral fusogenic ability to animal cells, modified the described adsorption properties. This is taken to mean that irreversible binding of Sendai viral particles to protoplast plasma membranes does occur, presumably via a different mechanism from that with animal cells. This should encourage studies aimed at testing the capacity of the reconstituted Sendai envelopes to serve as delivery carriers in plant genetic engineering projects.

Original languageEnglish
Pages (from-to)141-149
Number of pages9
JournalPlant Science
Volume41
Issue number2
DOIs
StatePublished - 25 Oct 1985

Keywords

  • concanavalin A
  • lectins
  • protoplast membranes
  • transformations

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