TY - JOUR
T1 - InterCells
T2 - A Generic Monte-Carlo simulation of intercellular interfaces captures nanoscale patterning at the immune synapse
AU - Neve-Oz, Yair
AU - Sajman, Julia
AU - Razvag, Yair
AU - Sherman, Eilon
N1 - Publisher Copyright:
© 2018 Neve-Oz, Sajman, Razvag and Sherman.
PY - 2018/9/11
Y1 - 2018/9/11
N2 - Molecular interactions across intercellular interfaces serve to convey information between cells and to trigger appropriate cell functions. Examples include cell development and growth in tissues, neuronal and immune synapses (ISs). Here, we introduce an agent-based Monte-Carlo simulation of user-defined cellular interfaces. The simulation allows for membrane molecules, embedded at intercellular contacts, to diffuse and interact, while capturing the topography and energetics of the plasma membranes of the interface. We provide a detailed example related to pattern formation in the early IS. Using simulation predictions and three-color single molecule localization microscopy (SMLM), we detected the intricate mutual patterning of T cell antigen receptors (TCRs), integrins and glycoproteins in early T cell contacts with stimulating coverslips. The simulation further captures the dynamics of the patterning under the experimental conditions and at the IS with antigen presenting cells (APCs). Thus, we provide a generic tool for simulating realistic cell-cell interfaces, which can be used for critical hypothesis testing and experimental design in an iterative manner.
AB - Molecular interactions across intercellular interfaces serve to convey information between cells and to trigger appropriate cell functions. Examples include cell development and growth in tissues, neuronal and immune synapses (ISs). Here, we introduce an agent-based Monte-Carlo simulation of user-defined cellular interfaces. The simulation allows for membrane molecules, embedded at intercellular contacts, to diffuse and interact, while capturing the topography and energetics of the plasma membranes of the interface. We provide a detailed example related to pattern formation in the early IS. Using simulation predictions and three-color single molecule localization microscopy (SMLM), we detected the intricate mutual patterning of T cell antigen receptors (TCRs), integrins and glycoproteins in early T cell contacts with stimulating coverslips. The simulation further captures the dynamics of the patterning under the experimental conditions and at the IS with antigen presenting cells (APCs). Thus, we provide a generic tool for simulating realistic cell-cell interfaces, which can be used for critical hypothesis testing and experimental design in an iterative manner.
KW - Agent based Monte-Carlo simulation
KW - Cell signaling
KW - Direct STORM
KW - Kinetic segregation model
KW - Microvilli
KW - Photoactivated localization microscopy
KW - Single molecule localization microscopy
KW - T cell activation
UR - http://www.scopus.com/inward/record.url?scp=85053108098&partnerID=8YFLogxK
U2 - 10.3389/fimmu.2018.02051
DO - 10.3389/fimmu.2018.02051
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 30254635
AN - SCOPUS:85053108098
SN - 1664-3224
VL - 9
JO - Frontiers in Immunology
JF - Frontiers in Immunology
IS - SEP
M1 - 2051
ER -