TY - JOUR
T1 - Intercommunication of DNA-Based Constitutional Dynamic Networks
AU - Yue, Liang
AU - Wang, Shan
AU - Lilienthal, Sivan
AU - Wulf, Verena
AU - Remacle, Françoise
AU - Levine, R. D.
AU - Willner, Itamar
N1 - Publisher Copyright:
© 2018 American Chemical Society.
PY - 2018/7/18
Y1 - 2018/7/18
N2 - Intercommunication between dynamic chemical networks plays a major role in cellular transformations. Inspired by nature, we introduce the intercommunication between two constitutional dynamic networks, CDNs, "S" and "T" composed, each, of four equilibrated supramolecular constituents AA′, AB′, BA′, and BB′, and of CC′, CD′, DC′, and DD′, respectively. Each of the constituents is conjugated to a Mg 2+ -ion-dependent DNAzyme unit that acts as a reporter element for the concentration of the respective constituent via the catalyzed cleavage of the fluorophore/quencher-functionalized substrate associated with the respective DNAzyme reporter. Also, constituents BB′ (in CDN "S") and CC′ (in CDN "T") include Mg 2+ -ion-dependent DNAzymes acting as activator units for generating triggering signals between the networks. Subjecting CDNs "S" and "T" to the catalytically cleavable hairpin trigger H dd′ or H aa′ , respectively, yields input strands that intercommunicate the CDNs by affecting the time-dependent re-equilibration of the constituents of the counter CDN without affecting the dynamic equilibrium of the constituents of the CDN that generates the triggering strands. Treatment of CDNs "S" and "T" with hairpins H dd′ and H aa′ (or H ba′ ), respectively, stimulates autonomous positive/positive or positive/negative feedback to the programmed time-dependent up-regulation or down-regulation of the equilibrated constituents in the two CDNs.
AB - Intercommunication between dynamic chemical networks plays a major role in cellular transformations. Inspired by nature, we introduce the intercommunication between two constitutional dynamic networks, CDNs, "S" and "T" composed, each, of four equilibrated supramolecular constituents AA′, AB′, BA′, and BB′, and of CC′, CD′, DC′, and DD′, respectively. Each of the constituents is conjugated to a Mg 2+ -ion-dependent DNAzyme unit that acts as a reporter element for the concentration of the respective constituent via the catalyzed cleavage of the fluorophore/quencher-functionalized substrate associated with the respective DNAzyme reporter. Also, constituents BB′ (in CDN "S") and CC′ (in CDN "T") include Mg 2+ -ion-dependent DNAzymes acting as activator units for generating triggering signals between the networks. Subjecting CDNs "S" and "T" to the catalytically cleavable hairpin trigger H dd′ or H aa′ , respectively, yields input strands that intercommunicate the CDNs by affecting the time-dependent re-equilibration of the constituents of the counter CDN without affecting the dynamic equilibrium of the constituents of the CDN that generates the triggering strands. Treatment of CDNs "S" and "T" with hairpins H dd′ and H aa′ (or H ba′ ), respectively, stimulates autonomous positive/positive or positive/negative feedback to the programmed time-dependent up-regulation or down-regulation of the equilibrated constituents in the two CDNs.
UR - http://www.scopus.com/inward/record.url?scp=85050233115&partnerID=8YFLogxK
U2 - 10.1021/jacs.8b03450
DO - 10.1021/jacs.8b03450
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C2 - 29965742
AN - SCOPUS:85050233115
SN - 0002-7863
VL - 140
SP - 8721
EP - 8731
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 28
ER -