Interferon inhibits prostaglandin E2 synthesis and stimulates (2'-5')oligoadenylate synthetase activity in peripehral blood mononuclear cells of inflammatory bowel disease patients

D. Rachmilewitz, F. Karmeli, A. Panet

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5 Scopus citations

Abstract

The effect of interferon (IFN) on prostaglandin E2 (PGE2) synthesis by peripheral blood mononuclear cells of normal subjects and patients with inflammatory bowel disease was studied. Exposure of peripheral blood mononuclear cells, isolated from normal subjects and patients with active ulcerative colitis and Crohn's disease, to IFN reduced the release of PGE2 in a dose-dependent manner. The inhibitory effect was observed for at least 24 h after the removal of IFN. To correlate the effect of IFN on PGE2 synthesis with a known activity of IFN, the induction of (2'-5')oligoadenylate synthetase (OAS) was also studied in the same mononuclear cells. Low basal levels of this enzyme were detected in mononuclear cells isolated from normal subjects and patients with active ulcerative colitis and Crohn's disease. Moreover, IFN treatment in vitro resulted in a similar induction of this enzyme in patients and controls. These results indicate that peripheral blood mononuclear cells of patients with active ulcerative colitis and Crohn's disease have normal response to IFN. In addition, our data do not suggest that IFN has an important role in the pathogenesis of the diseases.

Original languageEnglish
Pages (from-to)629-635
Number of pages7
JournalJournal of Interferon Research
Volume5
Issue number4
DOIs
StatePublished - 1985
Externally publishedYes

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